Elucidation of follicle selection system using bovine follicle culture and application for follicle and oocyte culture of wildlife

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For clarification of mechanisms of follicular development and degeneration, growing oocytes were collected from bovine and feline ovaries and cultured in vitro. It was suggested that high concentration of follicular stimulating hormone inhibited the oocyte growth in vitro, and estradiol was essential for oocyte and follicular development. Fibroblast growth factor 7 stimulated oocyte growth and granulosa cell proliferation in early culture stage, but it did not have previous effects on growing oocytes in late culture stage.