a Novel Screening System for Potential Anticancer Drugs with Gene Amplification Method

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DNA-binding molecules are potential anticancer drugs to regulate gene amplification or gene expressions. The accurate relative determination of DNA-binding affinities of chemical compounds is an important factor for drug discovery area. In this work, I have developed a novel system to evaluate relative DNA-binding affinities of chemical compounds with primer-dimer(PD) templates using a real-time PCR analyzer. The utility of PD templates allows us to be accurate and highly sensitive determination of DNA-binding affinities of chemical compounds without the amplification of non-specific PCR products. We also developed the colorimetric determination system of DNA-binding affinities of chemical compounds in combination with gold nanoparticle aggregation phenomena and PCR-based evaluation system described above.