Study on functions of biological clock during chondrogenesis using bioluminescence imaging

点击翻译按钮获取中文摘要

We have constructed bioluminescence imaging system for monitoring dual reporter expression at the single-cell level. Using this system, we succeeded in observing that bmal1 gene expression oscillates in anti-phase with per2 gene expression. In addition, bioluminescence imaging has showed that metabolic oscillations to exhibit coupled oscillations of ATP and oxygen are induced during chondrogenesis. We found that the metabolic oscillations are involved in prechondrogenic condensation, not in chondrogenic differentiation.