Research into the exchanges of the 0 antigen in Gram-negative bacteria

革兰氏阴性菌0抗原交换的研究

• 批准号: 21790424
• 负责人: IGUCHI Atsushi
• 金额: $ 2.75万
• 依托单位: University of Miyazaki (2010)Kobe University (2009)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21790424/
• 关键词: 遺伝; 0抗原; 大腸菌; 水平伝播; 157; O抗原

I investigated Escherichia. coli 0157 strains belonging to multiple lineages different from that of the 0157:H7 strains. Nucleotide sequences of the chromosomal region (about 59 kb) covering the 0157-antigen gene clusters and its flanking regions revealed that sequences of 0157-antigen gene clusters were divided into two distinct groups at sequence level. Interestingly, distribution of the two types of the 0157-antigen gene clusters did not follow the phylogenetic lineages of the strains, suggesting that the horizontal transfer of both types of 0157-antigen locus has occurred indipendently among E. coli strains. Additionally, we found the repeat sequences in the junction points of sequence similarity, suggesting that the repeat sequences were associated with the genomic rearrangement around the 0-antigen gene clusters.

我调查了埃希菌。大肠杆菌0157菌株与0157 H7菌株属于不同的多个谱系。覆盖0157-抗原基因簇及其两侧的染色体区域（约59 kb）的核苷酸序列显示，0157-抗原基因簇的序列在序列水平上分为两个不同的组。有趣的是，两种类型的0157抗原基因簇的分布并不遵循菌株的系统发育谱系，这表明两种类型的0157抗原位点的水平转移在大肠杆菌菌株中是独立发生的。此外，我们在序列相似性的连接点上发现了重复序列，表明重复序列与0抗原基因簇周围的基因组重排有关。