Analysis of gene expression of intracellular signal tansduction system in the heart failure model rats.

心力衰竭模型大鼠细胞内信号转导系统基因表达分析

• 批准号: 08671506
• 负责人: IGUCHI Atsushi
• 金额: $ 0.64万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08671506/
• 关键词: herat filure model; gene expression; signal transducion; ラット心臓; 圧負荷

Wister-Imamichi rats 3-4 weeks age were used for this study. Under aneasthesia, we established heart failure model rats by hemoclip located at the abdominal aorta above renal artery Control group was treated as same without hemoclip. Hearts were harvested i 2weeks after surgery and used for molecular biological analysis. Total RNAs were isolated from Wistar-Imamichi rat hearts using the acid guanidinium thyocianate/phenol/chloroform extraction. The total RNAs(40 mg) were electrophoresed on agarose gel and transferred onto a nylon membrane filter. RNA blotting analysis was performed using oligonucleotide probe for CaM kinase I, CaMkinase kinase, CaM kinase II, CaM kinase IV.There was no remarkable change in gene expression about these Ca/calmodulin dependent kinases between heart failure group and control group. This result suggests that CaM kinase I, CaMkinase kinase, CaM kinase II, CaM kinase IV had no significance in this heart failure model.

本研究使用3-4周龄的Wister-Imamichi大鼠。在麻醉状态下，于大鼠肾动脉上方腹主动脉夹闭建立心衰模型，对照组不夹闭。术后2周取心脏进行分子生物学分析。使用酸性胍/酚/氯仿提取从Wistar-Imamichi大鼠心脏分离总RNA。将总RNA（40 mg）在琼脂糖凝胶上电泳并转移到尼龙膜滤器上。用CaM激酶I、CaM激酶II、CaM激酶IV的寡核苷酸探针进行RNA印迹分析。心衰组与对照组相比，上述钙/钙调素依赖性激酶的基因表达无明显变化。提示CaM激酶I、CaM激酶II、CaM激酶IV在该心衰模型中无明显意义。