The role of Notch signaling in differentiation of human mast cells

我们试图阐明Notch信号在人肥大细胞分化中的作用。从人脐带血细胞中纯化出来的造血干细胞和祖细胞被涂膜Notch配体或逆转录病毒hes1转导刺激。缺口配体刺激不诱导肥大细胞分化。我们成功地实现了Notch信号分子的逆转录病毒转导，但对肥大细胞分化的影响仍在观察中。

We tried to clarify the role of Notch signaling in the differentiation of human mast cells. Hematopoietic stem cells and progenitors, purified from human cord blood cells, were stimulated by plate-coated Notch ligands, or retroviral transduction of Hes-1. Notch - ligand stimulation did not induce mast cell differentiation. We succeeded in retroviral transduction of Notch signaling molecules, but the effect on differentiation of mast cells were still under the observation.