Analysis of hematopoietic stem cell and leukemic stem cell niche by focusing on Spred-1 functions

本研究项目的目的是了解造血干细胞和白血病干细胞及其壁龛之间的异同。我使用Spred-1基因敲除和白血病小鼠骨髓细胞进行了竞争性再种群试验。白血病干细胞表现出与造血干细胞相似的细胞表面标记表型和多能性。此外，Spred-1敲除骨髓细胞的竞争性再种群试验表明，两种干细胞可能在其共享的生态位上竞争。然而，Spred-1敲除细胞不能显著拯救白血病小鼠。

The aim of this research project is to understand both the similarities and differences between hematopoietic stem cells and leukemic stem cells and their niches. I have carried out competitive repopulation assay using Spred-1 knockout and leukemia-developed mouse bone marrow cells. Leukemic stem cells showed cell surface marker phenotype and multipotency similar to that of hematopoietic stem cells. Furthermore, competitive repopulation assay with Spred-1 knockout bone marrow cells suggested that both stem cells might compete in their sharing niches. However, Spred-1 knockout cells could not significantly rescue the leukemia-developed mice.