The mechanistic analyses on the effects of ethano Ion the maturation and the differentiation of neural stem cells in the primordium of cerebral cortex.

乙醇离子对大脑皮层原基神经干细胞成熟和分化影响的机制分析

• 批准号: 21791035
• 负责人: TOCHITANI Shiro
• 金额: $ 2.75万
• 依托单位: The University of Tokushima
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21791035/
• 关键词: 脳発達障害; 神経前駆細胞; エタノール; GABAA受容体; 発生・分化; 神経幹細胞; GABA_A受容体; アルコール; 細胞分裂; 大脳皮質; 催奇形性; GABA; 細胞分化

Ethanol is a potent tetatogen to induce congenital malformation in the central nervous system. The results of this study showed that ethanol alters mitotic cleavage plane orientation of the neural progenitors at the apical surface of the ventricular zone in the developing neocortex. Immunohistochemical analyses also showed that the alpha4 subunit of GABA_A receptors is already expressed in the neural progenitors at E10. GABA and Taurine, the endogenous agonists of GABA_A receptors, are also present in the developing neocortex with specific laminar patterns throughout the stages after E10. These results suggest that fetal exposure to ethanol perturbs the regulation of mitotic spindle orientation via GABA_A receptors, which may result in congenital malformation in the central nervous system.

乙醇是诱发中枢神经系统先天性畸形的强毒性物质。本研究的结果表明，乙醇改变了发育中的新皮质中脑室区顶面的神经祖细胞的有丝分裂面方向。免疫组织化学分析也表明，GABA_A受体α 4亚基在E10时已经在神经祖细胞中表达。GABA_A受体的内源性激动剂GABA和牛磺酸也存在于发育中的新皮层中，在E10后的整个阶段具有特定的层状模式。这些结果表明，胎儿暴露于乙醇干扰有丝分裂纺锤体的方向调节通过GABA_A受体，这可能会导致先天性畸形的中枢神经系统。

项目成果

Yoshihiro Fukui Embryonic exposure to ethanol disturbs regulation of mitotic spindle orientation via GABAA receptors in neural progenitors in ventricular zone of developing neocortex.

Yoshihiro Fukui 胚胎接触乙醇会干扰发育中新皮质心室区神经祖细胞中 GABAA 受体对有丝分裂纺锤体方向的调节。

• 发表时间: 2010
• 期刊: Neuroscience letters 472
• 作者: Shiro Tochitani;Hiromi Sakata-Haga
• 通讯作者: Hiromi Sakata-Haga

発生期新皮質神経前駆細胞の細胞分裂軸制御に対するエタノールの影響

乙醇对新生皮质神经祖细胞细胞分裂轴调节的影响

• 发表时间: 2009
• 作者: 栃谷史郎;坂田ひろみ;福井義弘
• 通讯作者: 福井義弘

Embryonic exposure to ethanol disturbs regulation of mitotic spindle orientation via GABAA receptors in neural progenitors in ventricular zone of developing neocortex

• DOI: 10.1016/j.neulet.2010.01.071
• 发表时间: 2010-03-19
• 期刊: NEUROSCIENCE LETTERS
• 影响因子: 2.5
• 作者: Tochitani, Shiro;Sakata-Haga, Hiromi;Fukui, Yoshihiro
• 通讯作者: Fukui, Yoshihiro