Regulation of mesenchymal stem cells in vivo by endogenous electric fields

内源电场对体内间充质干细胞的调节

• 批准号: 21791755
• 负责人: MABUCHI Yo
• 金额: $ 2.75万
• 依托单位: Keio University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21791755/
• 关键词: 創傷治癒学; 間葉系幹細胞; 細胞移植; 再生医学; 移植・再生医療; 細胞・組織; 幹細胞制御

Wound healing is essential for maintaining the integrity of multicellular organisms. It is thought that Mesenchymal Stem Cells (MSCs) were concerned with wound healing, however because MSCs have only been isolated from tissue in culture, the equivalent cells have not been identified in vivo. In this study, we performed a comprehensive screening of putative surface markers in order to select those that are most predictive of a pure MSC population in hBM. The transplant of the human MSCs enabled to analyze the migration of the human MSCs after the injury in vivo. Isolation technology of human MSC can analyze pure stem cell itself and elucidation of wound healing by an imaging technology.

伤口愈合对于维持多细胞生物体的完整性至关重要。据认为，间充质干细胞（MSC）与伤口愈合有关，然而，由于MSC仅在培养中从组织中分离，因此尚未在体内鉴定出等效细胞。在这项研究中，我们进行了一个全面的筛选推定的表面标志物，以选择那些最能预测的纯骨髓间充质干细胞群体。人MSC的移植使得能够分析人MSC在体内损伤后的迁移。人MSC的分离技术可以通过成像技术分析纯干细胞本身和阐明伤口愈合。

项目成果

Mesp1+ early paraxial mesodermal cells supply initial bone marrow mesenchymal stem cells capable of differentiating into neural crest lineage cells.

Mesp1 早期近轴中胚层细胞提供能够分化为神经嵴谱系细胞的初始骨髓间充质干细胞。

• 发表时间: 2011
• 期刊: Inflammation and Regeneration 31(1)
• 作者: Niibe K;Morikawa S;Mabuchi Y;Araki D;Nakagawa T;Okano H;Matsuzaki Y
• 通讯作者: Matsuzaki Y

Prospective clonal isolation of Human Mesenchymal stem cells elucidates heterogeneity within the Multi-potent stem cell compartment

人间充质干细胞的前瞻性克隆分离阐明了多能干细胞室内的异质性

• 发表时间: 2010
• 作者: Yo Mabuchi;Kunimichi Niibe;他
• 通讯作者: 他

ヒト間葉系幹細胞の予期的分離とClonal解析

人间充质干细胞的预期分离和克隆分析

• 发表时间: 2010
• 作者: 森川暁、新部邦透;他
• 通讯作者: 他

The late effects of radiation on lifespan, lymphocyte proliferation and p53 haplodeficiency in mice

• DOI: 10.3109/09553002.2010.496031
• 发表时间: 2010-11-01
• 期刊: INTERNATIONAL JOURNAL OF RADIATION BIOLOGY
• 影响因子: 2.6
• 作者: Okazaki, Ryuji;Mabuchi, Yo;Norimura, Toshiyuki
• 通讯作者: Norimura, Toshiyuki

Isolation and Identification of Mesenchymal Stem Cells in Human Bone Marrow

人骨髓间充质干细胞的分离与鉴定

• 发表时间: 2009
• 作者: Mabuchi;Yo(発表者);Morikawa;Satoru;Suzuki;Sadafumi;Lein;Lawrence;Niibe;Kunimichi;Nagai;Yasuo;Sunabori;Takehiko;Okano;Hideyuki;Matsuzaki;Yumi;Yo Mabuchi;馬渕洋
• 通讯作者: 馬渕洋