Nobel bone regenerative medicine promoted by regulation of human MSC by Wnt/sFRP family

Wnt/sFRP家族调控人类MSC促进诺贝尔骨再生医学

• 批准号: 21791985
• 负责人: KATAGIRI Wataru
• 金额: $ 2.66万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21791985/
• 关键词: Wntシグナル; 骨再生医療; 幹細胞; MSC; sFRP-3; 未分化間葉系幹細胞; 組織工学; 細胞培養

Recently it has been revealed that Wnt signaling pathway regulates the osteoblastic differentiation of human mesenchymal stem cells (hMSCs). We used sFRP-3, the antagonist of Wnt, to regulate this signaling and investigated the effect on osteoblastic differentiation of hMSCs in vitro and in vivo. It was revealed that the addition of sFRP-3 showed early differentiation and mineralization of hMSCs, and also showed early bone regeneration in animal model. These findings indicated the possibility of novel bone regenerative medicine using sFRP-3.

近年来研究发现，Wnt信号通路调控人间充质干细胞（hMSCs）的成骨分化。我们利用Wnt拮抗剂sFRP-3调控这一信号通路，并在体外和体内研究了其对hMSCs成骨分化的影响。结果表明，在动物模型中，添加sFRP-3可使hMSCs早期分化和矿化，并表现出早期骨再生。这些发现表明利用sFRP-3进行骨再生医学的可能性。

项目成果

Involvement of the Wnt-β-catenin pathway in invasion and migration of oral squamous carcinoma cells

• DOI: 10.3892/ijo_00000761
• 发表时间: 2010-11-01
• 期刊: INTERNATIONAL JOURNAL OF ONCOLOGY
• 影响因子: 5.2
• 作者: Iwai, Soichi;Yonekawa, Atsuko;Yura, Yoshiaki
• 通讯作者: Yura, Yoshiaki

乳歯および永久歯歯髄細胞の遺伝子発現プロファイルの比較検討

乳牙和恒牙牙髓细胞基因表达谱的比较研究

• 发表时间: 2009
• 作者: 前薗葉月;野杁由一郎;朝日陽子;山口幹代;山本れいこ;薮根敏晃;上田未央;恵比須繁之;中村さやか
• 通讯作者: 中村さやか

Regulation of the Wnt signaling pathways during cell culture of human mesenchymal stem cells for efficient bone regeneration

人间充质干细胞培养过程中 Wnt 信号通路的调节以实现有效的骨再生

• 发表时间: 2010
• 期刊: Oral Science International (In press)
• 作者: Matsubara M;Yamachika E;Tsujigiwa H;Mizukawa N;Ueno T;Murakami J;Ishida N;Kaneda Y;Shirasu N;Takagi S.;Katagiri W
• 通讯作者: Katagiri W

ヒト乳歯歯髄由来幹細胞の細胞特性の検討Wnt/β-カテニン経路の関連

人乳牙牙髓干细胞的细胞特性检测与Wnt/β-catenin通路的关系

• 发表时间: 2009
• 作者: Kita K;Murakami J;et al;片桐渉
• 通讯作者: 片桐渉

Stem Cell Proliferation Pathways Comparison between Human Exfoliated Deciduous Teeth and Dental Pulp Stem Cells by Gene Expression Profile from Promising Dental Pulp

• DOI: 10.1016/j.joen.2009.07.024
• 发表时间: 2009-11-01
• 期刊: JOURNAL OF ENDODONTICS
• 影响因子: 4.2
• 作者: Nakamura, Sayaka;Yamada, Yoichi;Ueda, Minoru
• 通讯作者: Ueda, Minoru