Autologous transplantation of conjunctival epithelial stem cells

自体结膜上皮干细胞移植

• 批准号: 21659398
• 负责人: MIMURA Tatsuya
• 金额: $ 2.09万
• 依托单位: The University of Tokyo (2011)Okinaka Memorial Institute for Medical Research (2009-2010)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21659398/
• 关键词: 前駆細胞; 幹細胞; 角膜; 結膜

We cultured corneal and conjunctival epithelial cells from donor eye and rabbit eye. Additionally, we developed an adult precursor/stem cell isolation method(the sphere-forming assay) for corneal and conjunctival epithelial cells. We demonstrated corneal and conjunctival precursor/stem cells possessed the higher proliferative capacity as indicated by BrDU staining and the immature state as indicated by nestin staining compared with cultured differentiated epithelial cells. We cultured conjunctival epithelial cells on the denuded amniotic membrane and demonstrated that cultured conjuncdtival epithelium formed three to four layers on amniotic membrane. After transplantation of cultured conjunctival epithelial sheet on the rabbit cornea with corneal epithelial opacity and conjunctival invasion onto cornea, transplanted rabbit cornea maintained corneal clarity. These results suggest that transplantation of reconstructed conjunctival epithelial sheet using cultured conjunctival epithelial cells to the cornea is a possible treatment strategy for treating total limbal stem cell deficiency.

我们分别培养供眼和兔眼角膜和结膜上皮细胞。此外，我们开发了一种用于角膜和结膜上皮细胞的成体前体细胞/干细胞分离方法（球体形成试验）。我们证明，与培养的分化上皮细胞相比，BrDU染色显示角膜和结膜前体细胞/干细胞具有更高的增殖能力，巢蛋白染色显示未成熟状态。我们在羊膜上培养结膜上皮细胞，发现培养的结膜上皮在羊膜上形成3 ~ 4层。将培养的结膜上皮片移植到角膜上皮混浊、结膜浸润的兔角膜上后，移植兔角膜保持角膜清晰度。这些结果表明，利用培养的结膜上皮细胞将重建的结膜上皮片移植到角膜是治疗全角膜缘干细胞缺乏症的一种可能的治疗策略。

项目成果

Relationship between refractionand allergic conjunctivitis

屈光度与过敏性结膜炎的关系

• DOI: 10.1038/eye.2009.92
• 发表时间: 2009
• 期刊: Response to Dantas etal. Eye
• 作者: Honda N;Mimura T;Usui T;Amano S;Mimura T
• 通讯作者: Mimura T

Relationship betweenrefraction and allergic conjunctivitis

屈光与过敏性结膜炎的关系

• DOI: 10.1038/sj.eye.6702999
• 发表时间: 2009
• 期刊: Eye
• 影响因子: 3.9
• 作者: Mimura T;Mimura Y;Arimoto A;Amano S;Yamagami S;Funatsu H;Usui T;Noma H;Honda N;Okamoto S
• 通讯作者: Okamoto S

網膜静脈分枝閉塞症に伴う黄斑浮腫における術後の網膜感度と形態的検査との関連性

视网膜分支静脉阻塞合并黄斑水肿术后视网膜敏感性与形态学检查的关系

• 发表时间: 2011
• 作者: 野間英孝;船津英陽;張野正誉;三村達哉;堀貞夫
• 通讯作者: 堀貞夫

涙液中総IgE抗体価とアレルギー性結膜炎重症度の関係角膜カンファランス2011

泪液总 IgE 抗体滴度与过敏性结膜炎严重程度的关系 2011 年角膜会议

• 发表时间: 2011
• 作者: 保手濱充啓;三村達哉;森樹郎;臼井智彦;天野史郎
• 通讯作者: 天野史郎

外来でできる涙液・アレルギー検査

在门诊进行泪液/过敏测试

• 发表时间: 2010
• 作者: 保手濱充啓;三村達哉;森樹郎;臼井智彦;天野史郎;Tatsuya Mimura;Tatsuya Mimura;三村達哉
• 通讯作者: 三村達哉