Corneal regeneration using epithelial, stromal, and endothelial precursor cells based on cellular senescence control

我们开发了一种用于角膜上皮细胞、基质细胞和内皮细胞的成体干细胞=前体细胞分离方法（球体形成试验），该方法可以分离端粒长度、端粒酶活性和反映衰老特征差异的细胞。我们证明了使用上皮前体的角膜上皮片移植，使用角质细胞前体结合明胶水凝胶的基质片移植，或内皮前体球移植到角膜是一种可能的角膜再生治疗策略。

We developed an adult stem cell=precursor cell isolation method (the sphere-forming assay) for corneal epithelial, stromal, and endothelial cells, which could isolate cells with differences of telomere length, telomerase activity, and characteristics reflecting senescence. We demonstrated transplantation of corneal epithelial sheet using epithelial precursors, stromal sheet using keratocyte precursors combined with gelatin hydrogel, or spheres of endothelial precursors to the cornea is a possible treatment strategy for corneal regeneration.