Development of CLIP-RNAi method

RNAi是目前基因功能基础研究的重要实验工具。RNAi在各种生物技术和治疗应用中也有许多潜在的用途。我们最近开发了光诱导RNAi方法，称为CLIP-RNAi。在本研究中，我们改进了CLIP-RNAi的光反应性RNA载体。我们演示了CLIP-RNAi与几种可以在不同波长激发的荧光染料的使用。我们在体外和体内应用CLIP-RNAi方法。

RNAi is currently an important experimental tool for basic research into gene function. RNAi also has many potential uses in various biotechnological and therapeutic applications. We recently developed photoinducible RNAi method, termed CLIP-RNAi. In this study, we improved the photoresponsive RNA carrier for CLIP-RNAi. We demonstrate the use of CLIP-RNAi with several fluorescent dyes that can be excited at different wavelengths. We used CLIP-RNAi method in in vitro and in vivo applications.