Archaeal hunting : explore new metabolic functions of uncultured Archaea by using new culturing techniques

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Cultured enrichments and isolates are important in developing our understanding of microbial physiology, genetics, and ecology. However, many microorganisms, particularly members of the domain Archaea, remain uncultured. In this study, I attempted to cultivate and isolate uncultured Archaea from various environmental samples using new cultivation techniques I developed. As a result, I successfully cultivated and isolated methanogenic and methanotrophic Archaea. These results strongly suggest that the culturing methods I developed are effective ways to cultivate fastidious but ecologically important archaeal species that would otherwise escape conventional isolation strategies. Also, these isolates obtained in this study would promote to understand carbon cycle on earth and to develop new environmental technologies.