Development of a method for eva l uat i ng the host range of bacteriophages

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We developed a novel phage-host range evaluation method using EdU (5-ethynyl-2’-deoxyuridine) labeled phages, which consists of the following four steps. (i) Fluorescently labeled phages are added to a microbial consortium, and host cells are infected and fluorescently labeled. (ii) Fluorescent cells are sorted by FACS. (iii) 16S rRNA gene sequences retrieved from sorted cells are analyzed, and specific oligonucleotide probes for FISH are designed. (iv) Cells labeled with both fluorescently labeled phage and FISH probe are identified as host cells.