In vivo voltage-sensitive dye imaging of the neuronal activities in the mouse visual cortex evoked by visual and electrical stimuli

本研究利用电压敏感染料（VSD）对小鼠在体视皮层神经元活动进行光学测量，以评价小鼠视皮层神经元活动的时空特性。闪光灯施加到对侧眼诱导初级视觉皮层（V1）和纹状体外皮层（V2）的逐渐兴奋性反应。双相电流刺激V1诱发同步兴奋反应，随后在V1和V2的抑制反应。我们的研究结果表明，VSD成像在体内记录的活动与电生理记录的活动相当。

We conducted optical measurement of the mouse visual cortex activity in vivo using voltage-sensitive dye (VSD) to evaluate the spatio-temporal properties of the neuronal activities in the mouse visual cortex. A flash light applied to the contralateral eye induced gradual excitatory responses of the primary visual cortex (V1) and the extrastriatal cortex (V2). A biphasic current stimulation delivered to V1 evoked the synchronized excitatory responses followed by the inhibitory responses in the V1 and V2. Our results show that the activities recorded by the VSD imaging in vivo were comparable with that recorded by electrophysiological recording.