Creation of thermoresponsive nano-phase separated surfaces for cell adhesion and detachment

本研究的重点是通过在固体表面沉积温敏性嵌段共聚物来制备温敏性细胞培养表面，用于热调节细胞的粘附和脱离。温度响应性聚合物的接枝量、厚度和链长显著影响温控细胞粘附/脱离。通过优化这些因素，汇合培养的细胞被成功地收获为片状的细胞结构，通过降低温度。

This study focused on the fabrication of thermoresponsive cell culture surfaces through the deposition of thermoresponsive block copolymers on solid surfaces for thermally regulated cell adhesion and detachment. Grafted polymer amounts, thickness, and the chain length of thermoresponsive polymers significant affected the temperature-controlled cell adhesion/detachment. By optimizing these factors, confluently cultured cells were successfully harvested as sheet-like cellular architectures by reducing temperatures.