Establishment of a macaque cancer model using cancer cells derived from MHC homozygous macaque iPS cells

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We made somatic cells from iPS cells derived from cynomolgus macaque, which homozygously had a certain haplotype of major histocompatibility complex (Mafa-HT1). And then, we transduced oncogenes to the differentiated cells resulting in establishment of malignant cells, an embryonal carcinoma PTY and a glioblastoma NPC#5PR2. These two cancer cell lines could make tumors in NOG mouse. We transplanted PTY cellsinto subcutaneous, ovary and abdominal cavity of a cynomolgus macaque with heterogygous MHC. However, all PTY cells were rejected after 4 weeks post transplantation. On the other hand, we are transplanting NPC#5PR2 cells into an immune privilege site, brain, of a Mafa HT-1 heterozygous monkey.