Development of vitreous ice technique and correlative microscopy to observe biological specimens at a molecular resolution

我们尝试在获取断层重建的投影图像时，使用电容式液体倾斜仪测量倾斜角度。因此，测量的瓷砖角度与基于标记的对准具有相同的精度，而基于标记的对准是层析成像中最常用的对准方法。当投影图像中没有足够的基准标记时，测量倾斜角度将是获得更好层析图像的有效方法。

We attempted to gage the tilt angle using a capacitive liquid-based inclinometer when the projection images for tomographic reconstructionwere acquired. As a result, the tile angle measured has the same accuracy as those obtained by the marker-based alignment, which is most popular alignment method in tomography. When the enough fiducial markers are not available in the projection images, measuring the tilt angle will be the useful method to obtain the better tomogram.