Electron Spin Resonance Chemical Exchange Saturation Transfer Spectroscopy using Nitrogen Encapsulated Fullerene.

N@C60分子更容易展示ESR测量中的饱和现象，因为它的外例清晰ESR线。N@C60 molecule是一个很好的候选人，用于ESR化学交换饱和度转移光谱学。如果要获得一个水溶剂N@C60磁探测器，我们将N@C60与γ-cyclodextrin（N@C60·γ-CD）结合起来。然后我们成功地测量了水中羟基N@C60的ESR信号。我们确定了放松时间和N@C60与γ-CD在水中测量集中度和温度依赖性的N@C60的精细结构常数。我们也成功地获得了单晶体N@C60·γ-CD的X射线差异数据。从N@C60·γ-CD分子结构的准确信息中，我们可以确定电子和质子核自旋之间磁偶极相互作用的确切价值。We could confirm that the N@C60·\-CD complex is the best candidate for the magnetic probe of the ESR chemical exchange saturation transfer spectroscopy even in water。

N@C60 molecule easily exhibits a saturation phenomenon in ESR measurement, because of its extraordinarily sharp ESR line. N@C60 molecule is a good candidate for the magnetic probe of the ESR chemical exchange saturation transfer spectroscopy. In order to get a water soluble N@C60 magnetic probe, we synthesized the encapsulated complex of N@C60 with γ-cyclodextrin (N@C60・γ-CD). Then we succeeded to measure the ESR signal of the hydrophobic N@C60 in water. And we determined the relaxation time and the fine structure constants of N@C60 with γ-CD in water by measuring the concentration, and temperature dependence of ESR spectrum. We also succeeded to get the X-ray diffraction data of the single crystal N@C60・γ-CD. From the precise information of the N@C60・γ-CD molecular structure, we could determine the exact value of magnetic dipole interaction between electron and proton nuclear spins. We could confirm that the N@C60・γ-CD complex is the best candidate for the magnetic probe of the ESR chemical exchange saturation transfer spectroscopy even in water.