Evolution of the function maintenance system of plant organelle genome by C-to-U RNA editing

植物细胞器RNA编辑的催化分子长期以来一直未被确定。我们试图以拟南芥的psbE基因为模型，分离和鉴定这些分子。因此，我们用生化方法成功地浓缩了叶绿体裂解产物的催化活性，发现核糖核酸酶敏感成分(S)参与了催化反应。在此基础上，我们提出了一个C-to-U编辑机制的初步模型。

Catalytic molecules of the plant organellar RNA editing has long been unidentified. We attempted to isolate and characterize those molecules, using the psbE gene of Arabidopsis thaliana as a model. As a result, we succeeded in concentrating the catalytic activity from the chloroplast lysates by biochemical means, and found that RNase-sensitive component(s) should be involved in the catalytic reaction. Based on this we propose a tentative model of the C-to-U editing machinery.