SecDF function and roles of the proton motive force in protein translocation

在细菌中，蛋白质跨膜转运是由膜整合成分SecDF刺激的。然而，其分子机制仍不清楚。在本研究中，我们测定了嗜热嗜热菌SecDF在3.3A分辨率下的晶体结构，并根据其结构信息进行了一系列生化分析。最后，我们提出了一个工作假说，即SecDF作为一个膜集成马达，由PMF驱动，实现SEC机器的ATP非依赖的全蛋白质转运能力

In bacteria, protein translocation across the membrane is stimulated by membrane-integrated components, SecDF. However, itsmolecular mechanisms remainunknown. In this study, we determined the crystal structure of Thermus thermophilusSecDF at 3.3 A resolutionand carried out a number of biochemical analysis based on the structural information.Finally, we propose a working hypothesis that SecDF functions as a membrane-integrated motor, powered by PMF, to achieveATP-independent full protein translocation ability of the Sec machinery