Degradation mechanisms of sigma 32 by membrane targeting via SRP pathway

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In Escherichia coli.,a heat shock responsible transcription factor, sigma 32 is mainly and rapidly degraded by a membrane-bound ATP dependent protease, FtsH. However, precise molecular mechanisms of its degradation remain poorly understood. Using genetic analysis, Dr. Takashi Yura, one of our collaborators recently proposed that the SRP pathway involved in membrane protein biogenesis plays a significant role in the sigma 32 degradation. In this study, we carried out site-specific in vivo photo crosslinking analysis targeted to the sigma 32 and found that Ffh protein, a member of the SRP is in close proximity to the sigma 32.