Molecular mechanisms of pupal metamorphosis

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We have studied molecular mechanisms of pupal metamorphosis in Bombyx mori, specifically; (1) We determined using a singles celled Verson’s gland that the pupal commitment of one cell occurs gradually with various intermediate phases, which is different from the well known hypothesis. (2) Our preliminary studies showed that the decline in the competent of larval specific crochet forming cells, is due to nutrients by inactivating the insulin signaling pathway. (3) The cessation of JH biosynthesis in the last instar larvae is caused by several factors to work together to guarantee its cessation; such as the decline in ecdysone titer and the release of dopamine and sNPF. (4) The ecdysone inducible transcription factor BHR4 acts back to the prothoracic glands to suppress the ecdysone biosynthesis, so that this factor regulates the timing of pupal ecdysis.