Nematic ordered cellulose templates mediating 3 dimensional order-patterned deposition accompanied with synthesis of calcium phosphates

本研究试图提出一种利用模板的仿生矿化方法。它结合了形态学控制过程与合成磷酸钙作为骨骼的主要成分。修饰后的NOC模板成功介导了这一过程，该模板具有离子供应源和支架的双重作用，用于磷酸钙作为生物矿物的3d有序结构，具有生物矿化的关键功能。其次，有序的纤维素膜支架（NOC）也通过细胞附着和随后的培养诱导了表皮细胞三维分层结构的建立。此外，NOC膜作为半透模板，也被用来使表皮细胞层在垂直方向上三维增殖。该模板被证明适合作为三维细胞培养装置，因此提出这些三维细胞层的构建过程遵循皮肤形成的基本概念

This study attempts to propose a sortof biomimic mineralization using the template. It combines morphologically controlling process with synthesis of the calcium phosphate as a major component of bones. This process was successfully mediated by the modified NOC template having a pair of roles of the ion supply sources and scaffolds for 3D-ordering architecture of the calcium phosphate as a biomineral in the key functions for biomineralization. Secondly, the ordered cellulosefilm scaffold, NOC, also induced establishment of a three-dimensional, hierarchical structure of epidermal cells by cell attachment and subsequent culture. Furthermore, the NOC film, as a semipermeable template, was also employed to allow three-dimensional proliferation of epidermal cell layers in the perpendicular direction. The template provedto be a suitable as a three-dimensional cell culture device, resulting in the proposal that the construction processes of these three-dimensional cell layers followed the basic concept of skin formation