Nematic ordered cellulose templates mediating 3 dimensional order-patterned deposition accompanied with synthesis of calcium phosphates

本研究试图提出一种利用模板进行仿生矿化的方法。它将形态控制过程与作为骨骼主要成分的磷酸钙的合成相结合。这一过程成功地介导了改性的NOC模板具有一对作用的离子供应源和支架的三维有序结构的磷酸钙作为生物矿物的生物矿化的关键功能。其次，有序的纤维素膜支架，NOC，也诱导建立一个三维的，分层结构的表皮细胞的细胞附着和随后的培养。此外，NOC膜，作为一个半渗透性的模板，也被用来允许在垂直方向上的表皮细胞层的三维增殖。该模板被证明是一个适合作为三维细胞培养装置，从而提出这些三维细胞层的构建过程遵循皮肤形成的基本概念

This study attempts to propose a sortof biomimic mineralization using the template. It combines morphologically controlling process with synthesis of the calcium phosphate as a major component of bones. This process was successfully mediated by the modified NOC template having a pair of roles of the ion supply sources and scaffolds for 3D-ordering architecture of the calcium phosphate as a biomineral in the key functions for biomineralization. Secondly, the ordered cellulosefilm scaffold, NOC, also induced establishment of a three-dimensional, hierarchical structure of epidermal cells by cell attachment and subsequent culture. Furthermore, the NOC film, as a semipermeable template, was also employed to allow three-dimensional proliferation of epidermal cell layers in the perpendicular direction. The template provedto be a suitable as a three-dimensional cell culture device, resulting in the proposal that the construction processes of these three-dimensional cell layers followed the basic concept of skin formation