Elucidation of the interaction beween Babesia parasites and host erythrocytes

阐明巴贝虫寄生虫与宿主红细胞之间的相互作用

• 批准号: 22380154
• 负责人: YOKOYAMA Naoaki
• 金额: $ 12.81万
• 依托单位: Obihiro University of Agriculture and Veterinary Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010-04-01 至 2013-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22380154/
• 关键词: バベシア; 赤血球; 寄生; 制御性T細胞; 免疫回避; アクチン; スペリカルボディ; マイクロネーム; 膜; cytochrome P450; 遺伝子多型; マクロファージ; Gliding motility; Spherical body protein; 原虫; メロゾイト; 赤内型ステージ; バベシア原虫; 膜分子; 寄生戦略; Na,K-ATPase

This research project had been conducted to elucidate the interaction between Babesia parasites and host erythrocytes during their asexual growth. The findings are summarized below.1) Na, K-ATPase, an erythrocytic enzyme, is found on the surface of Babesia parasite. 2) A spherical body protein (SBP) expressed by Babesia parasite is released into the cytoplasm of the infected erythrocytes at the late phase of infection. 3) Micronemal proteins of Babesia parasites act as adhesion molecules to the receptors of host erythrocytes. 4) A surface antigen of Babesia parasite can interact with the erythrocytic actin during the infection. 5) Babesia parasite shows a unique "Gliding motility" during erythrocyte invasion. 6) Babesia parasite can escape from host immunity by activating host Treg cells. 7) Erythrocyte invasion of Babesia parasites can be inhibited by disturbing the molecular interactions between the protozoan ligands and host receptors.

该研究项目是为了阐明在无性增长期间巴比西亚寄生虫与宿主红细胞之间的相互作用。在下面总结了发现的结果。1）Na，K-ATPase，一种红细胞酶，在Babesia寄生虫的表面上发现。 2）在感染后期，由贝贝斯寄生虫表达的球体蛋白（SBP）释放到感染红细胞的细胞质中。 3）贝贝斯寄生虫的微生物蛋白充当宿主红细胞受体的粘附分子。 4）在感染期间，贝贝西亚寄生虫的表面抗原可以与红细胞肌动蛋白相互作用。 5）在红细胞入侵期间，巴贝西亚寄生虫显示出独特的“滑动运动”。 6）巴贝斯寄生虫可以通过激活宿主Treg细胞来摆脱宿主免疫。 7）通过干扰原生动物配体和宿主受体之间的分子相互作用，可以抑制红细胞寄生虫的红细胞侵袭。

项目成果

Molecular characterizations of a new spherical body protein of Babesia bovis and evaluation its potential use for serodiagnosis

牛巴贝虫新型球体蛋白的分子表征及其血清学诊断的潜在用途评估

• 发表时间: 2010
• 期刊: Jpn.J.Vet.Parasitol.
• 作者: Terkawi;M.A.;Seuseu;J.;AbouLaila;M.;Yokoyama;N.;Xuan;X.;Iearashi;I.
• 通讯作者: I.

MSA-1 gene-based genetic diversity of Babesia bovis detected in cattle populations in Thailand, Brazil, and Ghana.

在泰国、巴西和加纳的牛群中检测到基于 MSA-1 基因的牛巴贝斯虫遗传多样性。

• 发表时间: 2013
• 作者: 横山直明;長野大輔;T. Sivakumar;五十嵐郁男
• 通讯作者: 五十嵐郁男

Genetic diversity of merozoite surface antigens in Babesia bovis detected from Sri Lankan cattle

从斯里兰卡牛中检测到牛巴贝虫裂殖子表面抗原的遗传多样性

• DOI: 10.1016/j.meegid.2013.07.001
• 发表时间: 2013
• 期刊: Infect. Genet. Evol
• 作者: Sivakumar;T.;Okubo;K.;Igarashi;I.;de Silva;W.K.;Kothalawala;H.;Silva;S.S.;Vimalakumar;S.C.;Meewewa;A.S.;and Yokoyama;N
• 通讯作者: N

Evaluation of the in vitro growth-inhibitory effect of epoxomicin on Babesia parasites

• DOI: 10.1016/j.vetpar.2009.09.049
• 发表时间: 2010-01-20
• 期刊: VETERINARY PARASITOLOGY
• 影响因子: 2.6
• 作者: AbouLaila, Mahmoud;Nakamura, Kazuya;Igarashi, Ikuo
• 通讯作者: Igarashi, Ikuo

Inhibitory Effects of Pepstatin A and Mefloquine on the Growth of Babesia Parasites.

胃酶抑素 A 和甲氟喹对巴贝虫寄生虫生长的抑制作用。

• DOI: 10.4269/ajtmh.2012.12-0218
• 发表时间: 2012
• 期刊: American Journal of Tropical Medicine and Hygiene
• 影响因子: 3.3
• 作者: Munkhjargal T;Aboulaila M;Terkawi MA;Sivakumar T.;et al.
• 通讯作者: et al.