Establishment of a new alveolar epithelial cell model by gene transfection and its application to the study on drug transport and toxicity in the lungs
基因转染建立新型肺泡上皮细胞模型及其在肺部药物转运和毒性研究中的应用
基本信息
- 批准号:22390031
- 负责人:
- 金额:$ 11.32万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (B)
- 财政年份:2010
- 资助国家:日本
- 起止时间:2010-04-01 至 2014-03-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
In order to develop a new alveolar type II epithelial cell model, Abca3 gene was introduced into RLE-6TN cells. Compared to RLE-6TN cells, the expression of Abca3 mRNA and the formation of lamellar bodies, characteristic intracellular organelle in type II cells, were increased in RLE/Abca3 cells. Surprisingly, the expression of other type II marker mRNAs and albumin uptake activity were also increased, indicating that introduction of Abca3 gene would direct RLE-6TN cells to more type II-like cells. In addition, RLE/Abca3 cells responded well to TGF-beta1 and bleomycin, compounds inducing epithelial-mesenchymal transition in alveolar epithelial cells, than RLE-6TN cells. Thus, RLE/Abca3 cells may be a promising in-vitro model system to analyze and predict drug-induced lung toxicity.
为了建立一种新的肺泡II型上皮细胞模型,将Abca 3基因导入RLE-6 TN细胞。与RLE-6 TN细胞相比,RLE/Abca 3细胞中Abca 3 mRNA的表达和板层体(II型细胞的特征性细胞器)的形成增加。令人惊讶的是,其他II型标志物mRNA的表达和白蛋白摄取活性也增加,表明Abca 3基因的引入将引导RLE-6 TN细胞向更多的II型样细胞分化。此外,RLE/Abca 3细胞对TGF-β 1和博莱霉素的反应比RLE-6 TN细胞好,TGF-β 1和博莱霉素是诱导肺泡上皮细胞上皮-间充质转化的化合物。因此,RLE/Abca 3细胞可能是一个有前途的体外模型系统,以分析和预测药物诱导的肺毒性。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
ヒト由来肺胞上皮細胞A549におけるインスリン取り込みに及ぼす喫煙の影響解析
吸烟对人源肺泡上皮细胞A549胰岛素摄取的影响分析
- DOI:
- 发表时间:2011
- 期刊:
- 影响因子:0
- 作者:Machitani M;et al;前川眞見子;堀内大士
- 通讯作者:堀内大士
培養肺胞上皮細胞におけるインスリン輸送に及ぼすタバコ煙抽出物の影響
香烟烟雾提取物对培养肺泡上皮细胞胰岛素转运的影响
- DOI:
- 发表时间:2012
- 期刊:
- 影响因子:0
- 作者:湯元良子;堀内大士;小田啓祐;永井純 也;高野幹久
- 通讯作者:高野幹久
Expression and function of P-glycoprotein in alveolar epithelial cells and its modulation by cigarette smoke extract
肺泡上皮细胞P-糖蛋白的表达和功能及其烟气提取物的调节
- DOI:
- 发表时间:2013
- 期刊:
- 影响因子:0
- 作者:Ryoko Yumoto;Yoshihiro Sasaki;Taishi Horiuchi;Yuki Kato;Umi Kishimoto;Junya Nagai;Mikihisa Takano
- 通讯作者:Mikihisa Takano
Induction of phonotypical changes by transfection of a transporter gene Abca3 in cultured alveolar epithelial cell line RLE-6TN.
在培养的肺泡上皮细胞系 RLE-6TN 中转染转运蛋白基因 Abca3 诱导表型变化。
- DOI:
- 发表时间:2012
- 期刊:
- 影响因子:0
- 作者:Yamamoto;C.;Yumoto;R.;Sambuichi;K.;Oda;K.;Nagai;J. and Takano;M.
- 通讯作者:M.
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TAKANO Mikihisa其他文献
TAKANO Mikihisa的其他文献
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{{ truncateString('TAKANO Mikihisa', 18)}}的其他基金
Analysis of low temperature tolerance of membrane transporters and development of pan-transporter inhibitors
膜转运蛋白的低温耐受性分析及泛转运蛋白抑制剂的开发
- 批准号:
23659081 - 财政年份:2011
- 资助金额:
$ 11.32万 - 项目类别:
Grant-in-Aid for Challenging Exploratory Research
Research on the anticancer effects and multidrug-resistance modulating effects of Thai plants
泰国植物的抗癌作用和多重耐药性调节作用研究
- 批准号:
23406005 - 财政年份:2011
- 资助金额:
$ 11.32万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Study on the transport of proteins in alveolar type I/II epithelial cells for the development of new pulmonary DDS systems
研究肺泡 I/II 型上皮细胞中蛋白质的转运,以开发新型肺 DDS 系统
- 批准号:
19390043 - 财政年份:2007
- 资助金额:
$ 11.32万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Search for traditional medicines in Thailand and scientific evaluation of their pharmacological effects
寻找泰国传统药物并科学评价其药理作用
- 批准号:
19406004 - 财政年份:2007
- 资助金额:
$ 11.32万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Development of protein drug delivery system from the lung based on ligand recognition mechanisms of the receptors
基于受体配体识别机制的肺蛋白药物递送系统的开发
- 批准号:
17590125 - 财政年份:2005
- 资助金额:
$ 11.32万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Studies on the mechanisms underlying renal tubular deficiency due to the lack of the expression and function of CLCN5, a gene responsible for Dent's disease
CLCN5(一种导致 Dent 病的基因)表达和功能缺失导致肾小管缺陷的机制研究
- 批准号:
13672287 - 财政年份:2001
- 资助金额:
$ 11.32万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Search for the new, endogenous compound with multidrug resistance modulating activity and its application to cancer chemotherapy
寻找具有多药耐药调节活性的新型内源性化合物及其在癌症化疗中的应用
- 批准号:
11672169 - 财政年份:1999
- 资助金额:
$ 11.32万 - 项目类别:
Grant-in-Aid for Scientific Research (C)