An involvement of damage induced neuronal endopeptidase DINE in construction of neuro-muscular junction.

损伤诱导的神经元内肽酶 DINE 参与神经肌肉接头的构建。

• 批准号: 22390035
• 负责人: KIYAMA Hiroshi
• 金额: $ 11.9万
• 依托单位: Nagoya University (2011-2012)Osaka City University (2010)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22390035/
• 关键词: 脳・神経; プロテアーゼ; 神経筋接合部; 末梢神経; 軸索再生; 運動ニューロン; DINE; ECEL1; ノックアウト; トランスジェニック; DINE/ECEL1; シュワン細胞; DINE

We have identified nerve injury induced neuronal endopeptidase (DINE) using peripheral nerve injury model. DINE is induced in bothCNS and PNS neuronal cells in response to nerve injury. To reveal a function of this peptidase, we have made DINE knockout mice. These mice were lethal immediately after birth due to respiration problem. We identified that DINE-deficient mice exhibited the poor innervation of final branching of nerve and only a few neuromuscular junctions (NMJ), suggesting that DINE is a crucial molecule in distal axon arborization into muscle to establish NMJ. To reveal mechanisms underlying the phenotypes, we co-cultured DINE-deficient neurons and Schwann cells, and found that DINE-deficiency affected on axon-Schwann interaction and Schwann maturation. These data suggests that DINE is implicated in maturation of Schwann cells as well as proper construction of NMJ.

我们利用周围神经损伤模型鉴定了神经损伤诱导的神经元内肽酶(DINE)。神经损伤后，中枢神经系统和三叉神经节神经细胞均可诱导DINE。为了揭示这种肽酶的功能，我们让基因敲除小鼠进餐。由于呼吸问题，这些小鼠在出生后立即死亡。我们发现，Dine缺乏的小鼠表现出神经末梢分支的神经支配较差，仅有少数神经肌肉连接(NMJ)，这表明Dine是远端轴突分支到肌肉中建立NMJ的关键分子。为了揭示这些表型的机制，我们将缺餐神经元和雪旺细胞共同培养，发现缺餐影响轴突-雪旺相互作用和雪旺成熟。这些数据表明，Dine与雪旺细胞的成熟以及NMJ的正确构建有关。

项目成果

Pancreatitis- associated protein-I and pancreatitis associated protein-III expression in a rat model of kainic acid-induced seizure

红藻氨酸诱导癫痫大鼠模型中胰腺炎相关蛋白 I 和胰腺炎相关蛋白 III 的表达

• 发表时间: 2011
• 期刊: Neuroscience
• 影响因子: 3.3
• 作者: Kawahara S;Konishi H;Morino M;Ohata K;Kiyama H
• 通讯作者: Kiyama H

Analysis on expression of the regenerating gene (Reg) family members Reg-IIIb and Reg-IIIg in the mouse during development

小鼠发育过程中再生基因（Reg）家族成员Reg-IIIb和Reg-IIIg的表达分析

• 发表时间: 2011
• 期刊: J Comp Neurol
• 影响因子: 2.5
• 作者: Matsumoto S;Konishi H;Maeda R;Kiryu-Seo S;Kiyama H
• 通讯作者: Kiyama H

軸索再生の分子メカニズム

轴突再生的分子机制

• 发表时间: 2011
• 作者: 佐々木佳寛;岸本海;永井純也;湯元良子;高野幹久;木山博資
• 通讯作者: 木山博資

マウス個体発生におけるレクチン様タンパクReg-IIIβとReg-IIIγの発現解析

小鼠个体发育过程中凝集素样蛋白Reg-IIIβ和Reg-IIIγ的表达分析

• 发表时间: 2011
• 作者: 松本早紀子;小西博之;前田理亞;木山博資
• 通讯作者: 木山博資

Nerve Injury-Activated Microglia Engulf Myelinated Axons in a P2Y12 Signaling-Dependent Manner in the Dorsal Horn

• DOI: 10.1002/glia.21053
• 发表时间: 2010-11-15
• 期刊: GLIA
• 影响因子: 6.2
• 作者: Maeda, Mitsuyo;Tsuda, Makoto;Kiyama, Hiroshi
• 通讯作者: Kiyama, Hiroshi