Research for novel diagnostic marker of gynecologic tumors and establishment of rapid and low invasive detection system by super-targeting antibody.

妇科肿瘤新型诊断标志物研究及超靶向抗体快速低创检测体系的建立。

• 批准号: 22390118
• 负责人: KATO Kazunori
• 金额: $ 11.23万
• 依托单位: Toyo University (2011-2012)Juntendo University (2010)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22390118/
• 关键词: 癌; 腫瘍マーカー; 抗体; 診断薬; 婦人科腫瘍; 卵巣癌; 子宮体癌; EpCAM; TROP2; 癌幹細胞; エクソソーム; ビーズアレイ

We could succeed to generate the target antibody against the gynecologic cancers and to establish a high-sensitive detection system of novel tumor marker. The first, we generated mAb to human NCAM2 expressed on hormone-dependent prostate and breast cancer. In addition, we established seven mAbs against ovarian cancer and one to breast cancer. The second, in attempt to improve diagnosis for gynecologic cancers, we developed new sandwich ELISA and immunochromatography systems detecting human sEpCAM, sCD147, sEphA2 and sTROP2, respectively, with high sensitivity and specificity. The third, we examined the biomarker candidates that were predominantly expressed in cancer stem cells of endometrial cancers. Thus, this approach is feasible for non-invasive and high sensitive diagnosis for gynecologic cancers.

本研究有望成功制备抗妇科肿瘤的靶向抗体，建立高灵敏度的新型肿瘤标志物检测系统。第一，我们产生了抗人NCAM 2的mAb，该人NCAM 2在依赖于前列腺癌和乳腺癌上表达。此外，我们还建立了7个抗卵巢癌和1个抗乳腺癌的mAb。第二，为了改善妇科癌症的诊断，我们开发了新的夹心ELISA和免疫层析系统，分别检测人sEpCAM，sCD 147，sEphA2和sTR0P2，具有高灵敏度和特异性。第三，我们检查了主要在子宫内膜癌的癌症干细胞中表达的生物标志物候选物。因此，这种方法是可行的，无创和高敏感性诊断妇科癌症。

项目成果

Heme oxygenase-1 (HO-1) is constitutively up-regulated in top alpinists.

• DOI: 10.1016/j.bbrc.2011.11.064
• 发表时间: 2012-01
• 期刊: Biochemical and biophysical research communications
• 影响因子: 3.1
• 作者: Gota Miura;Kazunori Kato;Takahiko Shimizu;D. Shiga;T. Shirasawa

Stemness property of the human esophageal squamous cell carcinoma in three-dimensional culture.

三维培养人食管鳞状细胞癌的干细胞特性。

• 发表时间: 2012
• 作者: 藤原大介; 加藤和則 ;野原茂夫;松原久 昭;岩沼佳美;梶山美明
• 通讯作者: 梶山美明

Epigenetic modulation enhances therapeutic effect of anti-IL13R alpha 2 antibody in human mesothelioma xenografts

表观遗传调节增强抗 IL13R α2 抗体在人间皮瘤异种移植物中的治疗效果

• 发表时间: 2010
• 作者: Shioda N;Beppu H;Fukuda T;Li E;Kitajima I;Fukunaga K;竹之内信
• 通讯作者: 竹之内信

Butyrate inhibitis the Self-Renewal Capacity of Endometrial Tumor Side-Population Cells by Induction a DNA Damage Response.

丁酸盐通过诱导 DNA 损伤反应来抑制子宫内膜肿瘤侧群细胞的自我更新能力。

• 发表时间: 2011
• 期刊: Mol Cancer Ther.
• 作者: Kato K;Kuhara A;Yoneda T;Inoue T;Takao T;Ohgami T;Dan L;Kuboyama A;Kusunoki S;Takeda S;Wake N.Sodium
• 通讯作者: Wake N.Sodium

Epigenetic modulation enhances the therapeutic effect of anti-IL-13R(alpha)2 antibody in human mesothelioma xenografts

表观遗传调节增强抗 IL-13R(α)2 抗体在人间皮瘤异种移植物中的治疗效果

• 发表时间: 2011
• 期刊: Clin Cancer Res
• 影响因子: 11.5
• 作者: Takenouchi M;Hirai S;Sakuragi N;Yagita H;Hamada H;Kato K
• 通讯作者: Kato K