Establishment of salivary gland stem cell isolation, culture, and storage for the regeneration of salivary gland

这项研究的最终目标是通过细胞移植开发一种治疗唾液腺功能障碍的新方法。首先，我们建立了辐射致小鼠唾液腺萎缩模型。与培养的唾液上皮细胞或间充质干细胞的实验相似，单核细胞显著增加唾液分泌。为了解细胞移植修复唾液腺的机制，建立了体外唾液腺萎缩模型。本研究结果表明，移植细胞可能通过影响工资腺腺泡细胞分泌因子影响工资腺腺泡细胞的功能。

The final goal of this study is to develop a novel treatment for salivary gland dysfunction by cell transplantation. First, we established a mouse salivary gland atrophy model by irradiation. The administration of MNCs significantly increased saliva secretion as similar to the experiments with cultured salivary epithelial cells or mesenchymal stem cells. To understand the mechanism of salivary gland recovery by cell transplantation, in vitro salivary gland atrophy model was established. The results from this study have shown that the transplanted cells may affect the function of salary gland acinar cells via factors secreted from MNCs.