Effects of rotavirus vaccine on circulating strains in Africa

已经引进了安全有效的疫苗来保护儿童免受轮状病毒的影响，轮状病毒是全球儿童严重急性胃肠炎的主要原因。这项研究旨在通过分析马拉维布兰太尔哨兵医院收治的儿童的临床样本，为了解轮状病毒疫苗对流行的轮状病毒株的影响提供分子流行病学基础。这项研究表明，马拉维G8毒株在过去十年中通过与其他人类轮状病毒的基因重组而进化，改变了它们的VP4基因，同时保持了其余结构和非结构蛋白的保守基因型星座。对马拉维G12P[6]毒株和具有代表性的共循环毒株的分析为G12 VP7基因供体毒株与具有不同遗传背景的当代本地流通毒株经历的复杂阶段的重组事件提供了分子证据。

Safe and efficacious vaccines have been introduced to protect children from rotavirus, a major cause of severe acute gastroenteritis in children worldwide. This study aimed at providing a molecular epidemiologic basis on which to understand the effects of rotavirus vaccines on circulating wild-type rotavirus strains by analyzing clinical samples collected from children admitted to a sentinel hospital in Blantyre, Malawi. This study revealed that Malawian G8 strains evolved over the last decade through genetic reassortment with other human rotaviruses, changing their VP4 genotypes while maintaining conserved genotype constellations for the remaining structural and non-structural proteins. Analysis of Malawian G12P[6] strains and representative, co-circulating strains provided molecular evidence for complex stages of reassortment events that a G12 VP7 gene donor strain underwent with contemporary, locally-circulating strains possessing different genetic backgrounds.