Development of a quantitative and non-traumatic strategy to insert any nano-molecules into living cells with electrospray

开发定量和非创伤性策略，通过电喷雾将任何纳米分子插入活细胞中

• 批准号: 22500302
• 负责人: VIGOT Rejan
• 金额: $ 2.91万
• 依托单位: The Institute of Physical and Chemical Research
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22500302/
• 关键词: Electrospray; Transfection; Neuron; 電子スプレー; 遺伝子導入; 神経細胞; ナノバイオ; 脳神経疾患; 先端機能デバイス; Molecular and cellular neuroscience; Molecular and cellular neurobiology; Neuron, synapse, and neural circuit; Neural plasticity; Neurotransmitters and receptors

We designed a nanoelectrospray micronozzle to ionize, accelerate and focus molecules/particles toward a single neuron for injection. The two concentric capillaries with hydrophobic coating produced an electrospray of solutions, with localized deposition on solid target. Precise nozzle alignment and voltage wave form were both critical to achieve stable droplet production with peak current reaching 50-250nA. With anionic dye and epifluorescence microscopy, we observed particles reaching the gas-medium interface but also run-away droplets accumulation on the inside of the outer pipette, until an abrupt flooding of the tip and leak of solution. Various nozzle morphologies were tested but this limitation is intrinsic to the current nozzle. Improved designs based on simulations of electrostatic properties are presented.

我们设计了一种纳米电喷雾微喷嘴，用于将分子/粒子加速并聚焦到单个神经元上进行注射。具有疏水涂层的两个同心毛细管产生溶液的电喷雾，在固体靶上具有局部沉积。精确的喷嘴对准和电压波形对于实现峰值电流达到50- 250 nA的稳定液滴生产都是至关重要的。使用阴离子染料和落射荧光显微镜，我们观察到颗粒到达气体-介质界面，但也观察到在外部移液管内部积聚的逃逸液滴，直到尖端突然溢出和溶液泄漏。对各种喷嘴形态进行了试验，但这种限制是当前喷嘴固有的。提出了基于静电特性模拟的改进设计。