Analysis of deep microcirculation in cerebral cortex by Doppler optical coherence tomography

多普勒光学相干断层扫描分析大脑皮层深部微循环

• 批准号: 22500376
• 负责人: SEKI Junji
• 金额: $ 2.5万
• 依托单位: Kansai University (2012)National Cardiovascular Center Research Institute (2010-2011)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22500376/
• 关键词: ドップラOCT; 神経循環カップリング; 穿通枝血管; 機能的カラム; 層構造; 局所循環調節; 1次体性感覚野; 周波数領域OCT; ﾄﾞｯﾌﾟﾗOCT; 神経循環ｶｯﾌﾟﾘﾝｸﾞ; 脳微小循環; 血糖値; 脳機能イメージング

In order to visualize microvessels deeply embedded in the cortical tissue of brain, the Doppler frequency shift caused by blood flow is used as a marker to find out blood vessels in OCT signals. The 3-dimensional distribution of blood flow velocity obtained by Doppler OCT technique delineated penetrating microvessels as well as microvessels running deep in the cerebral cortex up to 1.5 mm depth. Penetrating arterioles were discriminated from venules based on the flow direction and pulsatility of blood flow. Electrical stimulus of hindpaw induced significant velocity increase in the penetrating arterioles (about 13% of the control value) and pial arterioles (10%) in the hindpaw area of primary somatosensory (S1) cortex, while the flow velocity did not change significantly in microvessels in the other area of S1 cortex. The information of structure and blood flow of microvessels in the direction of depth can help us to understand blood flow regulation among layers as well as functional columns of cerebral cortex.

为了更好地显示脑组织中的微血管，利用血流引起的多普勒频移作为标记，从OCT信号中识别出血管。通过多普勒OCT技术获得的血流速度的三维分布描绘了穿透性微血管以及在大脑皮层中深入至1.5 mm深度的微血管。根据血流的流动方向和脉动性将穿透性小动脉与小静脉区分开来。电刺激后爪可使初级体感皮层（S1）后爪区穿通小动脉和软膜小动脉的流速显著增加（分别为对照组的13%和10%），而S1皮层其他区域的微血管流速无明显变化。微血管在深度方向上的结构和血流信息可以帮助我们了解大脑皮层各层之间以及功能柱之间的血流调节。

项目成果

Temperature imaging of neural activity in rat brain evoked by whisker stimulation

胡须刺激引起的大鼠大脑神经活动的温度成像

• 发表时间: 2011
• 作者: 鈴木崇士;大井康浩;関淳二
• 通讯作者: 関淳二

マイクロチャネル内流れ中の血小板模擬粒子の運動

模拟血小板颗粒在微通道中流动期间的运动

• 作者: 田中慎之介;関淳二;板野智昭;関眞佐子
• 通讯作者: 関眞佐子

Visualization of microvessels deeply embedded in cerebral cortex of rats by Doppler optical coherence tomography

多普勒光学相干断层扫描对深埋于大鼠大脑皮层的微血管进行可视化

• 发表时间: 2011
• 期刊: Proc. 9th World Congress for Microcirculation, Medimond
• 作者: Seki J.;Suzuki T.;Ooi Y.
• 通讯作者: Ooi Y.

Effects of autonomic nervous system on functional neuroimaging: analyses based on the vector autoregressive model

自主神经系统对功能神经影像的影响：基于向量自回归模型的分析

• DOI: 10.1007/978-1-4614-1566-4_12
• 发表时间: 2012
• 期刊: Adv. Exp. Med. Biol.
• 作者: Seiyama A.;Sasaki Y.;Takatsuki A.;Seki J.
• 通讯作者: Seki J.

Visualization of microvessels deeply embedded in the rat cerebral cortex by Doppler OCT technique

利用多普勒 OCT 技术观察深埋于大鼠大脑皮层的微血管

• 发表时间: 2010
• 作者: Seki J.;Suzuki T.;Ooi Y.
• 通讯作者: Ooi Y.