Three-dimensional Analysis of Cerebral Microvessels and Nerve Cells with Optical Coherence Tomography

光学相干断层扫描脑微血管和神经细胞的三维分析

• 批准号: 13671479
• 负责人: SEKI Junji
• 金额: $ 2.5万
• 依托单位: National Cardiovascular Center Research Institute
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671479/
• 关键词: optical coherence tomography; cerebral cortex; microcirculation; neurovascular coupling; blood flow regulation; Doppler shift; functional column structure; 3-dimensional configuration

This research project was aimed to analyze 3-dimensional configuration of microvessels and nervous tissue in the cerebral cortex using optical coherence tomography (OCT) system, which is especially designed for in vivo imaging of biological tissues in our laboratory, and to clarify temporal and spatial coupling between neuronal activity and microcirculatory function.In the OCT system, superluminescent diodes (SLD) with central wavelength of 813 and 940 nm were used for low-coherence light source. The spatial resolutions of the system was evaluated by in vitro and in vivo measurements to be 16x14 and 8x17 μm^2 (longitudinal (depth) x lateral directions) for 813 and 940 nm, respectively. The penetration depth of the OCT system was 0.7-1 mm for the rat cerebral cortex.The OCT observation revealed that dura and without dura. The responses of the somatosensory cortices during electrical stimulation of the hind paw were also different among the preparation methods ; Delayed swelling of the cortical surface appeared in the somatosensory cortex following the stimulation in the case of dura removal, which was restricted to a thin surface layer with less than several 10 μm. It is considered to reflect the reactive hyperemia accompanying the neuronal activation.Analyzing Doppler frequency shift due to the blood flow in microvessels by the use of a short time Fourier transform (STFT), cross-sectional velocity profiles and their temporal changes were measured for the rat pial microvessels. While the central velocity in the pial arterioles varied largely with time following the heart beats, the velocity profiles nearly exhibited Poiseuille distribution. These results are consistent with theoretical prediction for the blood flow in microvessels with small Reynold's number and small Womersley parameter. The results of this study indicate that the OCT may provide 3-dimensional information of blood flow distribution in the cerebral cortex with high temporal resolution

本研究旨在利用本实验室专为生物组织活体成像设计的光学相干层析系统(OCT)分析大脑皮层微血管和神经组织的三维形态，阐明神经元活动与微循环功能之间的时间和空间耦合。该系统使用中心波长为813和940 nm的超辐射二极管(SLD)作为低相干光源。通过体外和体内测量，该系统的空间分辨率分别为16×14和8×17μm^2(纵向(深度)x横向)，分别为813 nm和940 nm。OCT系统对大脑皮层的穿透深度为0.7-1 mm，OCT观察显示有硬脑膜和无硬脑膜。不同制备方法对体感皮质电刺激后爪的反应也不同；去硬脑膜的体感皮层出现延迟肿胀，仅限于厚度小于10μm的薄层，反映了伴随着神经元激活的反应性充血。心搏动后，软脑膜小动脉的中心血流速度随时间变化很大，但速度曲线几乎呈泊松分布。这些结果与小雷诺数和小沃姆斯利参数的微血管内血流的理论预测是一致的。本研究的结果表明，OCT可以提供高时间分辨率的大脑皮质血流分布的三维信息

项目成果

Ono H, Sasaki Y, Bamba E, Seki J, Giddings JC, Yamamoto J: "Cerebral thrombosis and microcirculation of the rat during the oestrous cycle and after ovariectomy"Clin Exp Pharmacol Physiol.. 29(1-2). 73-78 (2002)

Ono H、Sasaki Y、Bamba E、Seki J、Giddings JC、Yamamoto J：“发情周期和卵巢切除后大鼠的脑血栓形成和微循环”Clin Exp Pharmacol Physiol.. 29(1-2)。

Sasaki Y, Yamamoto E, Seki J, Yamamoto J: "Ginkgo Biloba extract (EGB761) inhibits thrombus formation by He-Ne laser beam in cerebral vessels of stroke-prone spontaneously hypertensive rats"Microcirculation Annual. 17. 67-68 (2001)

Sasaki Y、Yamamoto E、Seki J、Yamamoto J：“银杏叶提取物 (EGB761) 通过 He-Ne 激光束抑制易发生中风的自发性高血压大鼠脑血管中的血栓形成”《微循环年鉴》。

Satomura Y, Seki J, Ooi Y, Yanagida T, Seiyama A: "In vivo imaging of the rat cerebral microvessels with optical coherence tomography"Clin Hemorheol Microcirc. (in press).

Satomura Y、Seki J、Ooi Y、Yanagida T、Seiyama A：“利用光学相干断层扫描对大鼠脑微血管进行体内成像”Clin Hemorheol Microcirc。

Seki J, Satomura Y: "Microcirculatory measurements with optical coherence tomography (in Japanese)"J Jap Soc Biorheology. 17(2). 67-74 (2003)

Seki J、Satomura Y：“利用光学相干断层扫描进行微循环测量（日语）”J Jap Soc Biorheology。

Seiyama A, Seki J, et al.: "Regulation of oxygen transport during brain activation : Stimulus-induced hemodynamic responses in human and animal cortices"Dynamic Medicine. 2. 6 (2003)

Seiyama A、Seki J 等人：“大脑激活过程中氧输送的调节：人类和动物皮质中刺激引起的血流动力学反应”动态医学。