The determination of optimal effective amount of the stretching in the cultured myoblast and elucidation of the mechanism of effects

点击翻译按钮获取中文摘要

We performed 15-minute relatively short extension stimulation in cultured myoblast (H9c2 cell strain, C2C12 cell strain) mechanically and checked the influence of proliferation and differentiation. In addition, we assayed the expression of the RNAs of MyoD family (MyoD, Myogenin, Myf5, MRF4) with the RT-PCR and Myogenin protein and Myosin protein expression with the Western Blotting method. As a result, stretch stimulation did not adversely affect the proliferation in the skeletal muscle reproduction process, and exerting a positive effect on differentiation was revealed. It was suggested that MyoD RNA , Myogenin RNA and Myf5 RNA and Myogenin protein and Myosin protein played an important role for this.