Establishment of assessment system for toxic chemicals based onabnormal protein recognition mechanism in eukaryotic cells.

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We focused accumulation of polyubiquitinated proteins in cells which reflect protein abnormality to evaluate chemical-induced toxicity with a new index. By analyzing changes in hardly-soluble polyubiquitin chainsdue to exposure to each test substance using six established cell lines, toxic chemicals could be divided into three groups in terms of their toxic effects on levels of cellular abnormal proteins, which were(1) increased in all cells(2) increased in a cell type-dependent manner(3) unchanged in all cellsThe present evaluation system is useful for safety management of chemicals based on the understanding of toxic mechanism.