Establishment of enrichmentculture of marine anammoxbacteria anddevelopment of anammox bio-reactor

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A higher nitrogen removal rate was attained by using column reactor enriched marine anammox bacteria inoculated with coastal sediment in Hiroshima bay. Microbial community structure revealed that at least two anammox species belonging to “Candidatus Scalindua” was present in the column reactor. The newly designed two probes could be used We also designed two oligonucleotide probes specific to each dominant “Candidatus Scalindua” species were designed and could visualize two species. The optimal temperature,salinity, and pH ranges for the one species were determined by batch experiments.