Structural bases for an expanded codon recognition by modified nucleotide.

已知tRNA反密码子第一个字母中的一些修饰核苷酸可以扩大密码子识别能力。本研究拟基于70年代核糖体中密码子-反密码子碱基的三维结构来揭示其作用机制。在本研究中，我成功地建立了一种可重复性制备高衍射率晶体的70年代核糖体的方法。一旦获得了良好的晶体，就需要在以下几个步骤中进行技术改进：细化结晶条件，减少收获过程中对晶体的破坏，以及在原子分辨率下进行数据收集的策略。

Some modified nucleotides in the first letter of a tRNA anticodon is known to expand the codon recognition ability. I planed to reveal the mechanism based on the three-dimensional structure of the codon-anticodon basepair in the 70S ribosome.In this study, I have succeeded to establish a reproducible method of 70S ribosome preparation for highly diffracted crystals. Once good crystals are obtained, technical improvements are required in the following steps: refinement of crystallization condition, less damage for crystals during harvesting, and strategy of data collection at an atomic resolution.