Novel peptide-saccharide conjugates for prevention of intestinal infection

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Preventive effects of glycomacropeptide (GMP) and ovomucin glycopeptide (OGP) against intestinal infection were investigated and the conjugates of GMP with xylooligosaccharide (XOS) and carboxymethyldextran (CMD) were prepared by the Maillard reaction to enhance the effect of GMP. Binding ability of GMP to intestinal pathogenic bacteria was evaluated by ELISA with biotinylated bacteria. GMP showed binding ability to Salmonella enteritidis (S.enteritidis) and enterohaemorrhic Escherichia coli O157:H7 (EHEC O157). OGP showed binding ability to EHEC O157. These binding abilities markedly decreased by sialydase treatment and were completely eliminated by penodate oxidation. These results indicated that carbohydrate moieties such as sialic acid in GMP and OGP are involved in binding to these bacteria. The effect of GMP on the adhesion and invasion of pathogenic bacteria to Caco-2 cells was also investigated. GMP was not potent inhibitor to adhesion and invasion of Salmonella infection. However, GMP-XOS and GMP-CMD significantly suppressed IL-8 production which was the index of infection. Our results indicate that GMP and OGP are promising as the agent to prevent intestinal infection.