High sensitive LC-MS/MS analysis of acylated anthocyanins for determination in bioanalytical samples

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It is well known that anthocyanins show excellent physiological functions. But it is unclear that anthocyanins play in living organisms, because they decompose easily in analysis, and there is no method to analyze anthocyanins high-sensitively in bioanalytical samples. Therefore, high sensitive LC-MS/MS analytical method of anthocyanins was developed. The limit of detection ranged from 0.05 to 1 pmol, resulted that the sensitivity was 100 times higher than those of HPLC. The recovery of anthocyanins from cell medium ranged from 70 to 140%. But the recovery from liver was0%, suggested the interference by ion suppression as phospholipid and so on.