Digestive physiology to development the artificial diet for sea urchin.

枯草酶和纤维素酶对羧甲基纤维素具有水解活性，最适温度为30℃，pH为8.0。对其热稳定性进行了表征，在pH 7.0条件下处理30 min后活性下降至50%的温度为32℃。在短棘海胆生长温度5 ~ 20℃时，分离得到的纤维素酶活性保持较高水平。因此，我们证实了短棘海胆的一种纤维素酶是适应低水温的酶。

Subtilase and cellulase exhibited hydrolytic activity toward carboxymethyl cellulose with optimum temperature and pH at 30 °C and pH 8.0, respectively. The thermal stability was characterized and temperature at which activity decreased to50% by the 30 min treatment at pH 7.0 was 32 °C. The isolated cellulase activity was remained at high level at 5-20 °C which is growth temperature of the short-spined sea urchin. Thus, we confirmed that a cellulase of the short-spined sea urchin is adaptiveenzyme around low water temperature.