Digestive physiology to development the artificial diet for sea urchin.

消化生理学开发海胆人工饲料。

• 批准号: 22580194
• 负责人: URA Kazuhiro
• 金额: $ 2.91万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22580194/
• 关键词: ウニ; 消化酵素; 精製; 特異抗体; 局在; 内臓; 免疫組織; 消化吸収; 人工餌料開発; プロテアアーゼ; 棘皮動物; セルラーゼ

Subtilase and cellulase exhibited hydrolytic activity toward carboxymethyl cellulose with optimum temperature and pH at 30 °C and pH 8.0, respectively. The thermal stability was characterized and temperature at which activity decreased to50% by the 30 min treatment at pH 7.0 was 32 °C. The isolated cellulase activity was remained at high level at 5-20 °C which is growth temperature of the short-spined sea urchin. Thus, we confirmed that a cellulase of the short-spined sea urchin is adaptiveenzyme around low water temperature.

枯草杆菌酶和纤维素酶对羧甲基纤维素具有水解活性，最适温度为30 °C，最适pH为8.0。热稳定性进行了表征，在pH7.0下处理30 min，活性下降至50%的温度为32 °C。分离的纤维素酶活性在短刺海胆生长温度5-20 °C下保持较高水平。因此，我们证实了短刺海胆的纤维素酶是低水温附近的适应酶。

项目成果

Purification and biochemical characterization of a cellulase from the digestive organs of the short-spined sea urchin Strongylocentrotus intermedius

短刺海胆中间型海胆消化器官纤维素酶的纯化和生化表征

• DOI: 10.1007/s12562-012-0528-y
• 发表时间: 2012
• 期刊: Fisheries Science
• 影响因子: 1.9
• 作者: Syuto Hasegawa;K. Ura;Hiroyuki Tanaka;T. Ojima;Y. Takagi
• 通讯作者: Y. Takagi

エゾバフンウニ消化管におけるスブチラーゼの局在解析。

海胆胃肠道枯草杆菌酶的定位分析。

• 发表时间: 2010
• 作者: K.Ura;S.Hasegawa;E.Tanaka;T.Gotoh;T.Ojima;Y.Takagi;長谷川嵩人・後藤孝弘・田中恵梨・尾島孝男・都木靖彰・浦和寛
• 通讯作者: 長谷川嵩人・後藤孝弘・田中恵梨・尾島孝男・都木靖彰・浦和寛

Purification and biochemical characterization of a cellulase from digestive organs of short-spined sea urchin Strongylocetrotus intermedius

短刺海胆 Strongylocetrotus intermedius 消化器官纤维素酶的纯化及生化表征

• 发表时间: 2012
• 期刊: Fish.Sci.
• 作者: S. Hasegawa;K. Ura;H. Tanaka;T. Ojima and Y. Takagi.;Syuto Hasegawa
• 通讯作者: Syuto Hasegawa

Immunohistochemical Analysis of the Proteolytic Enzyme Subtilase in the Digestive Organs of the Sea Urchin Strongylocentrotus intermedius

海胆中间圆甲藻消化器官中蛋白水解酶枯草杆菌酶的免疫组织化学分析

• DOI: 10.1002/ar.21484
• 发表时间: 2012
• 期刊: The Anatomical Record
• 作者: K.Ura;S.Hasegawa;E.Tanaka;T.Gotoh;T.Ojima;Y.Takagi
• 通讯作者: Y.Takagi