Characterization of immunotoxicological and immunopathological roles of fungal PAMPs

DBA/2是一株对真菌PAMPs-β-葡聚糖有较高反应的菌株。本研究对真菌PAMPs的免疫毒理学和免疫病理学作用进行了研究。结果表明，DBA/2小鼠在体外和体外均能产生高水平的炎性细胞因子，是一种高度敏感的品系。在体外研究中，DBA/2小鼠产生了显著高水平的炎性细胞因子，如GM-CSF、干扰素-γ和肿瘤坏死因子-α。它对细胞骨架的交联高度敏感，并受肌动蛋白聚合的干扰而调节。体内研究表明，产生高浓度的抗β-葡聚糖抗体并产生致死毒性。由此可见，这是分析β-葡聚糖介导的免疫毒理学反应的一个很好的工具。

DBA/2 is a strain, specifically higher response to fungal PAMPs, beta-glucan. In the present study, immunotoxicological and immunopathological rolesof fungal PAMPs were examined. From the results of this study, we have demonstratedthat DBA/2 mice is a highly sensitive strain to produce high level of inflammatory cytokinein vitro and in vito. In vitro study, DBA/2 mice produced significantly high level of inflammatory cytokines, such as GM-CSF, IFN-gamma, and TNF-alpha. It is highly sensitive tothe cross-linking of cytoskeleton and modulated by disrupting actin polymerization. In vivo study, producing high concentration of anti-beta-glucan antibody and induce lethal toxicity. From these findings, it is a good tool to analyze beta-glucan mediated immunotoxicological response.