Characterization of immunotoxicological and immunopathological roles of fungal PAMPs

真菌 PAMP 的免疫毒理学和免疫病理学作用的表征

• 批准号: 22590406
• 负责人: OHNO Naohito
• 金额: $ 3万
• 依托单位: Tokyo University of Pharmacy and Life Science
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22590406/
• 关键词: 感染免疫; PAMPs; 感染症; 自然免疫; βグルカン; αマンナン; 免疫毒性; マンナン; dectin-1; dectin-2; 肝臓; 遺伝子発現; apoptosis; 真菌; マイクロアレイ; 血管炎; 抗炎症作用; DBA/2マウス

DBA/2 is a strain, specifically higher response to fungal PAMPs, beta-glucan. In the present study, immunotoxicological and immunopathological rolesof fungal PAMPs were examined. From the results of this study, we have demonstratedthat DBA/2 mice is a highly sensitive strain to produce high level of inflammatory cytokinein vitro and in vito. In vitro study, DBA/2 mice produced significantly high level of inflammatory cytokines, such as GM-CSF, IFN-gamma, and TNF-alpha. It is highly sensitive tothe cross-linking of cytoskeleton and modulated by disrupting actin polymerization. In vivo study, producing high concentration of anti-beta-glucan antibody and induce lethal toxicity. From these findings, it is a good tool to analyze beta-glucan mediated immunotoxicological response.

DBA/2是一株对真菌PAMPs、β-葡聚糖特异性反应更高的菌株。本研究对真菌PAMPs的免疫毒理学和免疫病理学作用进行了研究。本研究结果表明，DBA/2小鼠是一个在体外和体内产生高水平炎性嘌呤的高度敏感品系。在体外研究中，DBA/2小鼠产生显著高水平的炎性细胞因子，如GM-CSF、IFN-γ和TNF-α。它对细胞骨架的交联高度敏感，并通过破坏肌动蛋白聚合来调节。在体内研究中，产生高浓度的抗β-葡聚糖抗体并诱导致死毒性。根据这些发现，它是分析β-葡聚糖介导的免疫毒理学反应的良好工具。

项目成果

Vasculitis and anaphylactoid shock in mice induced by the polysaccharide fraction secreted into culture supernatants by the fungus Candida metapsilosis

真菌化生念珠菌分泌到培养上清液中的多糖组分诱导小鼠血管炎和过敏性休克

• DOI: 10.1111/j.1348-0421.2011.00326.x
• 发表时间: 2011
• 期刊: Microbiol Immunol
• 影响因子: 2.6
• 作者: Rui Tada;Yusuke Takano;Hisashi Murakami;Ken-ichi Ishibashi;Noriko Nagi-Miura;Yoshiyuki Adachi;Naohito Ohno
• 通讯作者: Naohito Ohno

カンジダマンナンによる血管炎モデルにおける構造要求性について

念珠菌甘露聚糖诱导的血管炎模型的结构要求

• 作者: TH.Hida;K. Ishibashi;NN. Miura;Y. Adachi;N. Ohno;Ohno N;平田尚人;佐藤亘
• 通讯作者: 佐藤亘

Disruption of Actin Cytoskeleton Enhanced Cytokine Synthesis of Splenocytes Stimulated with Beta-Glucan from the Cauliflower Medicinal Mushroom, Sparassis crispa Wulf.:Fr. (Higher Basidiomycetes) in Vitro

• DOI: 10.1615/intjmedmushr.v14.i3.30
• 发表时间: 2012-01-01
• 期刊: INTERNATIONAL JOURNAL OF MEDICINAL MUSHROOMS
• 影响因子: 1.2
• 作者: Shibata，Asami;Hida，Toshie H.;Ohno，Naohito
• 通讯作者: Ohno，Naohito

Candida albicans可溶性多糖画分CAWS投与マウス肝臓における遺伝子発現の網羅的解析

白色念珠菌可溶性多糖组分 CAWS 处理小鼠肝脏基因表达的综合分析

• 发表时间: 2011
• 作者: 大野尚仁;大村崇;三浦典子;石橋健一;安達禎之
• 通讯作者: 安達禎之

Augmentation of cytokine synthesis of splenocytes stimulated with soluble β-Glucan in vitro by inhibiting actin polymerization

通过抑制肌动蛋白聚合来增强体外可溶性 β-葡聚糖刺激的脾细胞的细胞因子合成

• 发表时间: 2011
• 作者: Naohito Ohno;Asami Shibata;Toshie H Hida;Ken-Ichi Ishibashi;Noriko N Miura;Yoshiyuki Adachi
• 通讯作者: Yoshiyuki Adachi