植物模式识别受体（PRRs）可识别病原物的病原相关分子模式（PAMPs），从而激发免疫反应。已报道多种植物病原细菌和真菌的PAMPs/PRRs识别模式，但还未发现植物线虫的蛋白类PAMPs及PRRs。我们前期用植物受体CERK1为诱饵蛋白，通过酵母双杂获得与CERK1互作的拟禾本科根结线虫翻译延长因子Mg-eEF1A，并用生物信息学分析获得与细菌的PAMPs—EF-TU同源的爪哇根结线虫线粒体翻译延长因子MjEF-TU。实验表明它们均可诱导寄主防卫基因上调，暗示它们可能是根结线虫的PAMPs。本项目拟研究Mg-eEF1A和MjEF-TU的分泌特性、对PTI反应的影响、诱导植物抗性及作用通路等，从而明确它们的PAMPs特性及触发植物免疫反应的机制。本项目提供一个鉴定植物线虫PAMPs的新方法，首次发现植物线虫的蛋白类PAMPs及PRRs，为今后利用PAMPs/PRRs防治植物线虫奠定基础。

Molecules of Plant pathogens, i.e. pathogen associated molecular patterns (PAMPs), can be recognized by host cell surface localized pattern-recognition receptors (PRRs), which would trigger plant immunity. Currently, several recognition mode from plant bacteria and fungi between PAMPs and PRRs have been characterized. However proteinaceous PAMPs from plant-parasitic nematodes (PPNs) and PRRs haven’t been found yet. Before the project, one elongation factor Mj-eEF1A from Meloidogyne graminicola was found to interact with the PRR CERK1 by using yeast two-hybrid system. Based on this, the other elongation factor MjEF-TU from PPNs was found to match the bacteria PRR EF-TU by using bioinformatics analysis. Moreover, the experiments showed that Mg-eEF1A and MjEF-TU can induce up-regulation of several defense gene expressions in host plants, suggesting that Mg-eEF1A and MjEF-TU are possibly PAMPs of root-knot nematodes. Therefore, the following research contents of this project would be: to analyze the influences of Mg-eEF1A and MjEF-TU on PTI; to study the secretion characteristics of Mg-eEF1A and MjEF-TU; to study their characteristics of induction immunity and action pathway in plants, etc.; then to demonstrate whether Mg-eEF1A and MjEF-TU from Meloidogyne are PAMPs, and their recognition mode with the PRRs. The present project is the first time to provide a new method to get the PPNs PAMPs and it is hopeful to obtain the first proteinaceous PAMPs from PPNs and PRRs, laying foundation for controlling PPNs by using PAMPs/PRRs.