Pathophysiologial role of Helicobacter pylori asparaginase

幽门螺杆菌天冬酰胺酶的病理生理作用

• 批准号: 22590410
• 负责人: SHIBAYAMA Keigo
• 金额: $ 2.33万
• 依托单位: National Institute of Infectious Diseases
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22590410/
• 关键词: ヘリコバクターピロリ; アスパラギナーゼ; 代謝酵素; 病原性; 感染成立; アスパラギン取り込み; 免疫回避; 胃がん; ピロリ菌; 胃癌; 胃炎; 胃潰瘍; アスパラギン代謝

Helicobacter pylori sparaginase exhibited striking cytotoxic activity against U937 cells via asparagine deprivation. The cytotoxic activity of live H. pylori cells against U937 cells was significantly diminished by deletion of the asparaginase gene. An asparaginase-deficient mutant strain was significantly less capable of colonizing Mongolian gerbils. H. pylori cells incorporated extracellular asparagine by transporting aspartate produced by the action of the asparaginase. H. pylori asparaginase may be involved in inhibition of normal lymphocyte function at the gastric niche, allowing H. pylori to evade the host immune system.

幽门螺杆菌门冬酰胺酶通过天冬酰胺剥夺对U937细胞具有显著的细胞毒活性。活H. pylori细胞对U937细胞的杀伤作用因天冬酰胺酶基因的缺失而显著减弱。天冬酰胺酶缺陷的突变株是显着不太能够殖民蒙古沙鼠。H.幽门螺杆菌细胞通过转运由天冬酰胺酶作用产生的天冬氨酸而掺入细胞外天冬酰胺。H.幽门螺杆菌门冬酰胺酶可能参与抑制胃内正常淋巴细胞功能，使H. pylori以逃避宿主免疫系统。

项目成果

Molecular Epidemiology of Helicobacter cinaedi isolates in Japan.

日本胃螺杆菌分离株的分子流行病学。

• 发表时间: 2011
• 作者: Rimbara E;Shibayama K
• 通讯作者: Shibayama K

Role of gamma-glutamyl transpeptidase and asparaginase in the pathogenesis of Helicobacter pylori

γ-谷氨酰转肽酶和天冬酰胺酶在幽门螺杆菌发病机制中的作用

• 发表时间: 2013
• 作者: Liu J;Wu P;Gao F;Qi J;Kawana-Tachikawa A;Xie J;Vavricka CJ;Iwamoto A;Li T;Gao GF.;林原 絵美子,森 茂太郎,金 玄,柴山 恵吾
• 通讯作者: 林原 絵美子,森 茂太郎,金 玄,柴山 恵吾

Molecular epidemiologic analysis and antimicrobial resistance of Helicobacter cinaedi isolated from 7 hospitals in Japan

日本7家医院猪螺杆菌分子流行病学分析及耐药性

• 发表时间: 2012
• 作者: Ando H;Kitao T;Miyoshi-Akiyama T;Kato S;Mori T;Kirikae T.;E. Rimbara
• 通讯作者: E. Rimbara

Pathophysiological Role of Helicobacter pylori Asparaginase.

幽门螺杆菌天冬酰胺酶的病理生理学作用。

• 发表时间: 2011
• 作者: Shibayama K;Takeuchi H;Wachino J;Mori S;Arakawa Y
• 通讯作者: Arakawa Y

Biochemical and pathophysiological characterization of Helicobacter pylori asparaginase.

幽门螺杆菌天冬酰胺酶的生化和病理生理学特征。

• DOI: 10.1111/j.1348-0421.2012.00459.x
• 发表时间: 2012
• 期刊: Microbiology and Immunology
• 影响因子: 2.6
• 作者: Nakayama K;Nakamura H;Koga M;Koibuchi T;Fujii T;Miura T;Iwamoto A;Kawana-Tachikawa A.;Shibayama K
• 通讯作者: Shibayama K