Influence of prior pandemic A(H1N1)2009 virus infection on invasion of MDCK cells by community-acquired MRSA

先前大流行A(H1N1)2009病毒感染对社区获得性MRSA侵袭MDCK细胞的影响

• 批准号: 22590514
• 负责人: TAKAYAMA Yoko
• 金额: $ 2.58万
• 依托单位: Kitasato University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22590514/
• 关键词: A(H1N1)pdm09; community-acquired MRSA; invasion; 新型インフルエンザ; 市中感染型MRSA; 細胞侵入性; MRSA; 細胞接着能

In this study, we investigated whether prior infection with pandemic A(H1N1)2009 virus (A(H1N1)pdm09) led to increased invasion of airway epithelial cells by CA-MRSA. We performed the gentamicin killing assay using Madin-Darby canine kidney (MDCK) cells and various bacteria to investigate whether prior A(H1N1)pdm09 infection led to increased invasion of these epithelial cells by CA-MRSA. We found that the invasion rate of two MRSA strains (ATCC BAA-1680 (USA 300) and ATCC BAA-1699 (USA 100)) into intact MDCK cell monolayers was 0.29±0.15% and 0.007±0.002%, respectively (p=3). In addition, the relative invasion rate of both ATCC BAA-1680 and ATCC BAA-1699 was significantly increased by prior A(H1N1)pdm09 infection of MDCK monolayers from 1±0.28 to 1.38±0.02 and from 1±0.24 to 1.73±0.29, respectively (p

在这项研究中，我们调查了先前感染2009年甲型H1N1大流行病毒(A(H1N1)pdm09)是否导致CA-MRSA增加对呼吸道上皮细胞的侵袭。我们利用马丁达比犬肾(MDCK)细胞和各种细菌进行庆大霉素杀伤实验，以研究先前感染A(H1N1)pdm09是否导致CA-MRSA对这些上皮细胞的侵袭增加。结果发现，ATCC BAA-1680(USA300)和ATCC BAA-1699(USA100)对完整的MDCK细胞单层的侵袭率分别为0.29±0.15%和0.007±0.002%(P=3)。甲型H1N1亚型pdm09感染MDCK后，ATCC BAA-1680和ATCC BAA-1699的相对侵袭率分别从1±0.28和1±0.24增加到1.38±0.02和1.73±0.29(P

项目成果

市中感染型MRSAの気道上皮培養細胞への侵入性に関する検討

社区获得性MRSA侵入培养的气道上皮细胞能力的研究

• 发表时间: 2010
• 作者: 矢野寿一;平潟洋一;Lynch SV;Kunishima H;大島英敏;Yamashita H;矢野寿一;矢野寿一;平潟洋一;矢野寿一;矢野寿一;平潟洋一;平潟洋一;堀亨;Yano H;Arai K;Endo S;Yano H;矢野寿一;Kanamori H;Kanamori H;平潟洋一;國島広之;矢野寿一;青柳哲史;遠藤史郎;Kanamori H;Hatta M;Kanamori H;Kunishima H;Kanamori H;Yano H;Kuroda H;Kanamori H;堀亨;Hirakata Y;矢野寿一;高山陽子
• 通讯作者: 高山陽子