Application of rapid gene analyzing method for susceptibility to cancer

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MDM2 is known as an oncoprotein that binds to p53 protein and inactivates the tumor suppressor activity of p53. Genetic polymorphisms in the MDM2 gene are suggested to be a tumor susceptibility marker and a prognostic factor for cancer. In this study, we have developed a new SNP detection method applied Smart Amplification Process (SmartAmp) method to detect the SNP c.309T>G in the MDM2 gene and conducted a large-scale case-controlled study. We found that there were no significant associations between MDM2 SNP309 genotypes and lung cancer risk. Subgroup analysis of smoking related lung cancer indicated that G/G genotype of SNP309 may be associated with lung cancer carcinogenesis.