Study for correlation between Sarcoidosis and Propionibacteria and its application to diagnostic method

结节病与丙酸杆菌相关性研究及其在诊断方法中的应用

• 批准号: 15590489
• 负责人: KATAOKA Mikio
• 金额: $ 2.24万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15590489/
• 关键词: Sarcoidosis; Propionibacterium acnes; Propionibacterium granulosum; Bronchoalveolar lavage; Real-time PCR; Propoinibacterium acnes; リアルタイム PCR

Aim : Despite the extensive investigations into the pathogenesis of sarcoidosis, its etiology is still unknown. We have reported alveolar lymphocytes from patients with sarcoidosis are sensitized by Propionibacterium acnes (P.acnes) and the proliferation and IL-2 production in these cells were seen when these cells were stimulated by P.acnes. Recently, Eishi and his colleague have reported that many genomes of P.acnes and Propionibacterium granulosum (P.granulosum) were detected in lymph nodes from sarcoidosis patients. In this study, we investigated the quantitative analysis of Propionibacterial DNA in bronchoalveolar lavage (BAL) cells in patients with sarcoidosis to clarify the role of Propionibacteria in the pathogenesis of sarcoidosis. Moreover, we investigated the serum level of antibody.Subjects and Methods : Bronchoalveolar lavage cells were obtained from 42 patients with sarcoidosis and from those with 30 non-sarcoid lung diseases. BAL cells were undergone assays of real-time … More PCR for Propionibacterial DNA in Light Cycler apparatus using Taq Man probes and primers to amplify 16S rRNA of Propionibacteria. DNA was extracted using QlAamp DNA Mini Kit and the concentration of DNA was measured in the spectrophotometer. The number of bacterial genomes in 500 ng of total DNA were estimated with an internal standard curve for each species. The serum level of antibody against P.acnes was estimated using whole bacterial ELISA method. The titer of antibody was shown as optical density.Results : The number of genomes of P.acnes in BAL cells in patients with sarcoidosis are higher than in those with non-sarcoid lung diseases. However, there was no difference in the number of genomes of P.granulosum in BAL cells between sarcoidosis patients and non-sarcoidosis patients. There are clear relationship between the number of genomes of P.acnes in BAL cells and serum angiotensin converting enzyme level and proportion of macrophage and lymphocyte in BAL fluids in patients with sarcoidosis. The antibody level against P.acnes in patients with sarcoidosis was significantly higher than in those with non-sarcoid lung diseases.Conclusion : These results suggest that P.acnes might be involved in the pathogenesis of sarcoidosis. Less

目的：尽管结节病的发病机制已被广泛研究，但其病因仍不清楚。我们已经报道了结节病患者的肺泡淋巴细胞被痤疮丙酸杆菌（P.acnes）致敏，并且当这些细胞被痤疮丙酸杆菌刺激时，观察到这些细胞中的增殖和IL-2产生。最近，Eishi和他的同事报道了在结节病患者的淋巴结中检测到许多痤疮丙酸杆菌和颗粒丙酸杆菌的基因组。在这项研究中，我们研究了定量分析支气管肺泡灌洗（BAL）细胞中的丙酸杆菌DNA结节病患者，以澄清丙酸杆菌在结节病的发病机制中的作用。对象和方法：收集42例结节病患者和30例非结节病患者的支气管肺泡灌洗液细胞，用免疫组化法检测血清抗体水平。对BAL细胞进行实时荧光定量分析， ...更多信息 使用TaqMan探针和引物在Light Cycler装置中对丙酸杆菌DNA进行PCR，以扩增丙酸杆菌的16 S rRNA。使用QIAamp DNA Mini Kit提取DNA，并在分光光度计中测量DNA的浓度。用每个物种的内标曲线估计500 ng总DNA中的细菌基因组数量。采用全菌ELISA法检测痤疮丙酸杆菌抗体水平。结果：肺结节病患者支气管肺泡灌洗液中痤疮丙酸杆菌的基因组数目明显高于非肺结节病患者。但结节病患者和非结节病患者的BAL细胞中颗粒变形杆菌的基因组数量无差异。结节病患者支气管肺泡灌洗液中痤疮丙酸杆菌基因组数目与血清血管紧张素转换酶水平及支气管肺泡灌洗液中巨噬细胞和淋巴细胞比例有明显关系。结节病患者血清中抗痤疮丙酸杆菌抗体水平明显高于非结节病患者。结论：痤疮丙酸杆菌可能参与了结节病的发病过程。少

项目成果

Propionibacterium acnes DNA detected in bronchoalveolar lavage cells from patients with sarcoidosis.

• 发表时间: 2003-10
• 期刊: Sarcoidosis, vasculitis, and diffuse lung diseases : official journal of WASOG
• 作者: J. Hiramatsu;M. Kataoka;Y. Nakata;K. Okazaki;S. Tada;M. Tanimoto;Y. Eishi

サルコイドーシス.エクセルナース[免疫アレルギー編]

结节病。 Excel 护士 [免疫学和过敏版]

• 发表时间: 2004
• 作者: Ishida Tetsuo;Tetsuo Ishida;Atsushi Hirano;Hirohisa Ichikawa;Mikio Kataoka;片岡幹男
• 通讯作者: 片岡幹男

サルコイドーシス患者肺胞リンパ球のPropionibacterium acnesとの反応

结节病患者肺泡淋巴细胞对痤疮丙酸杆菌的反应

• 发表时间: 2003
• 期刊: 日本サルコイドーシス/肉芽種性疾患学会誌 23
• 作者: 片岡幹男;中田安成
• 通讯作者: 中田安成

Sarcoidosis

• DOI: 10.3760/cma.j.issn.1673-4904.2009.31.001
• 发表时间: 2009-11
• 作者: 陶仲为

Sarcoidosis occurring after interferon-α therapy for chronic hepatisis C.

干扰素-α 治疗慢性肝炎后发生的结节病 C.

• 发表时间: 2005
• 期刊: Respirology 10
• 作者: Ishida Tetsuo;Tetsuo Ishida;Atsushi Hirano
• 通讯作者: Atsushi Hirano