The relation between changes of ApoE-rich HDL subclasses and reactivity to HDL-C homogenous assay

高密度脂蛋白(高密度脂蛋白)有几个亚类，它们富含或缺乏载脂蛋白E(富含载脂蛋白E或缺乏载脂蛋白E的高密度脂蛋白)。我们利用Native2维凝胶电泳法研究了高密度脂蛋白亚类在短期和长期储存过程中的分布和稳定性。研究结果表明：1)富含载脂蛋白E的高密度脂蛋白由大颗粒和小颗粒组成。2)在37℃下放置4h后，小颗粒转变为大颗粒。3)长期存放4℃后，小颗粒减少或增多，大颗粒略有增加。这些发现表明，LCAT活性可能会在储存过程中改变富含ApoE的高密度脂蛋白亚类的组成。

High-density lipoprotein (HDL) has several subclasses which abundantly or poorly contain apolipoprotein E (ApoE-rich or ApoE-poor HDL). We investigated the distribution and stability of HDL subclasses during the short-term or long-term storage using native2-dimensional gel electrophoresis. The findings are as follows, 1) ApoE-rich HDL consists of the large- and small-particles. 2) Small-particles were converted into large-particles after 4h-storage at 37℃. 3) Small-particles were decreased or increased, and large-particleswere slightly increased after long-term storage even at 4℃. These findings suggest that LCAT activity may change the composition of ApoE-rich HDL subclasses during storage.