The relation between changes of ApoE-rich HDL subclasses and reactivity to HDL-C homogenous assay

高密度脂蛋白（HDL）有几个亚类，含有丰富或缺乏载脂蛋白E（ApoE-丰富或ApoE-穷人HDL）。我们采用天然双向凝胶电泳技术研究了HDL亚类在短期和长期储存过程中的分布和稳定性。结果表明：（1）富含ApoE的HDL由大颗粒和小颗粒组成; 2)在37℃下放置4 h后，小颗粒转化为大颗粒。3)即使在4℃下长期贮存，小颗粒物也有减少或增加的趋势，大颗粒物略有增加。这些研究结果表明，LCAT活性可能会改变富含ApoE的HDL亚类的组成在存储过程中。

High-density lipoprotein (HDL) has several subclasses which abundantly or poorly contain apolipoprotein E (ApoE-rich or ApoE-poor HDL). We investigated the distribution and stability of HDL subclasses during the short-term or long-term storage using native2-dimensional gel electrophoresis. The findings are as follows, 1) ApoE-rich HDL consists of the large- and small-particles. 2) Small-particles were converted into large-particles after 4h-storage at 37℃. 3) Small-particles were decreased or increased, and large-particleswere slightly increased after long-term storage even at 4℃. These findings suggest that LCAT activity may change the composition of ApoE-rich HDL subclasses during storage.