The relation between changes of ApoE-rich HDL subclasses and reactivity to HDL-C homogenous assay

高密度脂蛋白（HDL）有几个亚类，它们含有丰富或缺乏载脂蛋白E（富含载脂蛋白E或缺乏载脂蛋白E的HDL）。我们利用原生二维凝胶电泳技术研究了高密度脂蛋白亚类在短期和长期储存中的分布和稳定性。结果表明：1)富含apoe的HDL由大颗粒和小颗粒组成。2) 37℃贮藏4h后，小颗粒转化为大颗粒。3)即使在4℃长期存放，小颗粒也有所减少或增加，大颗粒略有增加。这些发现表明，LCAT活性可能改变储存过程中富含apoe的HDL亚类的组成。

High-density lipoprotein (HDL) has several subclasses which abundantly or poorly contain apolipoprotein E (ApoE-rich or ApoE-poor HDL). We investigated the distribution and stability of HDL subclasses during the short-term or long-term storage using native2-dimensional gel electrophoresis. The findings are as follows, 1) ApoE-rich HDL consists of the large- and small-particles. 2) Small-particles were converted into large-particles after 4h-storage at 37℃. 3) Small-particles were decreased or increased, and large-particleswere slightly increased after long-term storage even at 4℃. These findings suggest that LCAT activity may change the composition of ApoE-rich HDL subclasses during storage.