Autologous transplantation of neural stem cells in neonatalhypoxic ischemic brain injury in rats

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In this study, neural stem/neural progenitor cells with strongmitotic ability were obtained from the ischemic brain lesion at 3 says after neonatalhypoxic ischemic insult in rats. However, aspiration of ischemic tissue from neonatalbrain led to expand of ischemic lesions on MRI image and worsen of motor function test.Therefore, we changed the therapeutic method of this study from autologoustransplantation to enhancement intrinsic neural stem/ neural progenitor cells in thesubventricular zone (SVZ) and ischemic lesions with intraventricular injection of EGFand FGF which were usually used on neurosphere culture method. This method led to increasebromodeoxyuridine (BrdU) labeled cells in the SVZ, lower edge of corpus callosum andaround of ischemic lesion. Histological investigation of brain tissue at 7days afterhypoxia ischemia, almost all BrdU labeled cells were double labeled with neural progenitormaker DCX, astrocyte maker GFAP and oligodendrocyte progenitor maker O4, respectively.Therefore, BrdU labeled cells were seemed to still undifferentiated stage in thisanalysis.