Effects of neurokinin-1 receptors in monocytes and cell-to-cell interaction on intraluminal thrombus generation

P物质(SP)通过神经激肽-1受体(NK1R)激活单核细胞，促进全血凝血，但其确切机制尚不清楚。观察SP和NK1R拮抗剂Spantide对人单核细胞THP-1释放微粒的影响。柠檬化的人血浆与FITC结合的纤维蛋白原混合，作为单核细胞来源的促凝活性的标志。用荧光显微镜观察了实验性静脉血栓的生长情况。SP激活THP-1可增加具有促凝血活性的组织因子微粒的数量。Spantide显著抑制THP-1微球的释放。在THP-1存在的情况下，静脉模型中血栓的生长显著促进。

Substance-P (SP) enhances whole blood coagulation by activation of monocytes through neurokinin-1 receptors (NK1R) while the precise mechanisms of this phenomenon remain to beestablished. Effects of SP and a NK1R antagonist Spantide on the release of microparticles from human monocytic cells THP-1 were evaluated. Citrated human plasma, mixed with FITC-conjugated fibrinogen, was used for the marker of monocyte-derived procoagulant activity. The growth of thrombi generated in an experimental model for vein was also evaluated by fluorescence microscopy. Activation of THP-1 by SP increased the number of microparticles possessing tissue factor with procoagulant activity. Spantide significantly suppressed the release of microparticles from THP-1. The growth of thrombi in the vein model was significantly enhanced in the presence of THP-1.