Effects of neurokinin-1 receptors in monocytes and cell-to-cell interaction on intraluminal thrombus generation

单核细胞中的神经激肽-1受体和细胞间相互作用对管腔内血栓形成的影响

• 批准号: 22591748
• 负责人: AZMA Toshiharu
• 金额: $ 2.5万
• 依托单位: Saitama Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22591748/
• 关键词: 周術期管理学; 静脈血栓塞栓症; 単球; ニューロキニン1受容体; サブスタンスP; 血液凝固; 組織因子; マイクロパーティクル; ニューロキニン1 受容体; 白血球; 単球・マクロファージ; 深部静脈血栓; 血小板; 単球・マクロフージ

Substance-P (SP) enhances whole blood coagulation by activation of monocytes through neurokinin-1 receptors (NK1R) while the precise mechanisms of this phenomenon remain to beestablished. Effects of SP and a NK1R antagonist Spantide on the release of microparticles from human monocytic cells THP-1 were evaluated. Citrated human plasma, mixed with FITC-conjugated fibrinogen, was used for the marker of monocyte-derived procoagulant activity. The growth of thrombi generated in an experimental model for vein was also evaluated by fluorescence microscopy. Activation of THP-1 by SP increased the number of microparticles possessing tissue factor with procoagulant activity. Spantide significantly suppressed the release of microparticles from THP-1. The growth of thrombi in the vein model was significantly enhanced in the presence of THP-1.

P物质（SP）通过神经激肽-1受体（NK 1 R）激活单核细胞而增强全血凝固，但这一现象的确切机制仍有待建立。评价了SP和NK 1 R拮抗剂Spantide对人单核细胞THP-1释放微粒的影响。将与FITC结合的纤维蛋白原混合的柠檬酸盐人血浆用于单核细胞源性促凝血活性的标志物。还通过荧光显微镜评价了静脉实验模型中产生的血栓的生长。通过SP激活THP-1增加了具有促凝血活性的组织因子的微粒的数量。Spantide显著抑制微粒从THP-1的释放。在静脉模型中血栓的生长在THP-1的存在下显著增强。

项目成果

予定脊椎手術患者の血栓強度に対する麻酔管理法の影響

麻醉管理方法对择期脊柱手术患者血栓强度的影响

• 发表时间: 2013
• 作者: Shu H;Arita H;Hayashida M;Zhang L;An K;Huang W;Hanaoka K;東 俊晴
• 通讯作者: 東 俊晴

Detection of the full-length transcript variant for neurokinin-1 receptor in human whole blood associated with enhanced reinforcement of clot by substance-P

检测人全血中神经激肽 1 受体的全长转录变体，与 P 物质增强凝块强化相关

• DOI: 10.1007/s11239-011-0650-1
• 发表时间: 2012
• 期刊: J Thromb Thrombolysis 2012
• 作者: Azma T;Sugimoto Y;Kinoshita H;Ito T;Tsukamoto M;Hoshijima H;Nakao M;Kikuchi H
• 通讯作者: Kikuchi H

Anesthesia 21 Centuryニューロキニン受容体と血栓形成

麻醉 21 世纪神经激肽受体和血栓形成

• 发表时间: 2010
• 作者: 浦部晶夫;島田和幸;川合眞一編集;東 俊晴,伊藤大真,杉本由紀
• 通讯作者: 東 俊晴,伊藤大真,杉本由紀